Solution structure of DnaE intein from Nostoc punctiforme: Structural basis for the design of a new split intein suitable for site-specific chemical modification

Jesper S. Oeemig, Sesilja Aranko, Janica Djupsjöbacka, Kimmo Heinämäki, Hideo Iwaï*

*Corresponding author for this work

Research output: Contribution to journalArticleScientificpeer-review

71 Citations (Scopus)

Abstract

Naturally split DnaE intein from Nostoc punctiforme (Npu) has robust protein trans-splicing activity and high tolerance of sequence variations at the splicing junctions. We determined the solution structure of a single chain variant of NpuDnaE intein by NMR spectroscopy. Based on the NMR structure and the backbone dynamics of the single chain NpuDnaE intein, we designed a functional split variant of the NpuDnaE intein having a short C-terminal half (C-intein) composed of six residues. In vivo and in vitro protein ligation of model proteins by the newly designed split intein were demonstrated.

Original languageEnglish
Pages (from-to)1451-1456
Number of pages6
JournalFEBS Letters
Volume583
Issue number9
DOIs
Publication statusPublished - 6 May 2009
MoE publication typeA1 Journal article-refereed

Keywords

  • Chemical modification
  • Intein
  • NMR spectroscopy
  • Nuclear spin relaxation
  • Protein ligation
  • Protein splicing
  • Protein trans-splicing

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