Solid-support immobilization of a "swing" fusion protein for enhanced glucose oxidase catalytic activity

Research output: Contribution to journalArticleScientificpeer-review

Researchers

  • Yoshiyuki Takatsuji
  • Ryota Yamasaki
  • Atsushi Iwanaga
  • Michael Lienemann
  • Markus Linder

  • Tetsuya Haruyama

Research units

  • VTT Technical Research Centre of Finland
  • Kyushu Institute of Technology
  • Japan Science and Technology Agency

Abstract

The strategic surface immobilization of a protein can add new functionality to a solid substrate; however, protein activity, e.g., enzymatic activity, can be drastically decreased on immobilization onto a solid surface. The concept of a designed and optimized "molecular interface" is herein introduced in order to address this problem. In this study, molecular interface was designed and constructed with the aim of attaining high enzymatic activity of a solid-surface-immobilized a using the hydrophobin HFBI protein in conjunction with a fusion protein of HFBI attached to glucose oxidase (GOx). The ability of HFBI to form a self-organized membrane on a solid surface in addition to its adhesion properties makes it an ideal candidate for immobilization. The developed fusion protein was also able to form an organized membrane, and its structure and immobilized state on a solid surface were investigated using QCM-D measurements. This method of immobilization showed retention of high enzymatic activity and the ability to control the density of the immobilized enzyme. In this study, we demonstrated the importance of the design and construction of molecular interface for numerous purposes. This method of protein immobilization could be utilized for preparation of high throughput products requiring structurally ordered molecular interfaces, in addition to many other applications.

Details

Original languageEnglish
Pages (from-to)186-191
Number of pages6
JournalColloids and Surfaces B: Biointerfaces
Volume112
Publication statusPublished - 1 Dec 2013
MoE publication typeA1 Journal article-refereed

    Research areas

  • Glucose oxidase, Hydorphobin, Self-organized membrane, Surface functionalization

ID: 4809243