Single-molecule kinetics and super-resolution microscopy by fluorescence imaging of transient binding on DNA origami

Research output: Contribution to journalArticle

Researchers

  • Ralf Jungmann
  • Christian Steinhauer
  • Max Scheible
  • Anton Kuzyk

  • Philip Tinnefeld
  • Friedrich C. Simmel

Research units

  • Technische Universität München
  • Ludwig Maximilian University of Munich
  • Institute of Operating Systems and Computer Networks

Abstract

DNA origami is a powerful method for the programmable assembly of nanoscale molecular structures. For applications of these structures as functional biomaterials, the study of reaction kinetics and dynamic processes in real time and with high spatial resolution becomes increasingly important. We present a single-molecule assay for the study of binding and unbinding kinetics on DNA origami. We find that the kinetics of hybridization to single-stranded extensions on DNA origami is similar to isolated substrate-immobilized DNA with a slight position dependence on the origami. On the basis of the knowledge of the kinetics, we exploit reversible specific binding of labeled oligonucleotides to DNA nanostructures for PAINT (points accumulation for imaging in nanoscale topography) imaging with <30 nm resolution. The method is demonstrated for flat monomeric DNA structures as well as multimeric, ribbon-like DNA structures.

Details

Original languageEnglish
Pages (from-to)4756-4761
Number of pages6
JournalNano Letters
Volume10
Issue number11
Publication statusPublished - 10 Nov 2010
MoE publication typeA1 Journal article-refereed

    Research areas

  • biophysics, DNA origami, fluorescence microscopy, Nanobiotechnology, single-molecule kinetics, super-resolution

ID: 30234673