Self-Assembly of Minimal Peptoid Sequences

Valeria Castelletto*, Jani Seitsonen, Kunal M. Tewari, Abshar Hasan, Robert M. Edkins, Janne Ruokolainen, Lalit M. Pandey, Ian W. Hamley, King Hang Aaron Lau

*Corresponding author for this work

Research output: Contribution to journalArticleScientificpeer-review

1 Citation (Scopus)
7 Downloads (Pure)

Abstract

Peptoids are biofunctional N-substituted glycine peptidomimics. Their self-assembly is of fundamental interest because they demonstrate alternatives to conventional peptide structures based on backbone chirality and beta-sheet hydrogen bonding. The search for self-assembling, water-soluble "minimal" sequences, be they peptide or peptidomimic, is a further challenge. Such sequences are highly desired for their compatibility with biomacromolecules and convenient synthesis for broader application. We report the self-assembly of a set of trimeric, water-soluble α-peptoids that exhibit a relatively low critical aggregation concentration (CAC ∼0.3 wt %). Cryo-EM and angle-resolved DLS show different sequence-dependent morphologies, namely uniform ca. 6 nm wide nanofibers, sheets, and clusters of globular assemblies. Absorbance and fluorescence spectroscopies indicate unique phenyl environments for ?-interactions in the highly ordered nanofibers. Assembly of our peptoids takes place when the sequences are fully ionized, representing a departure from superficially similar amyloid-type hydrogen-bonded peptide nanostructures and expanding the horizons of assembly for sequence-specific bio- and biomimetic macromolecules.

Original languageEnglish
Pages (from-to)494-499
Number of pages6
JournalACS Macro Letters
Volume2020
Issue number9
DOIs
Publication statusPublished - 19 Mar 2020
MoE publication typeA1 Journal article-refereed

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    Castelletto, V., Seitsonen, J., Tewari, K. M., Hasan, A., Edkins, R. M., Ruokolainen, J., ... Lau, K. H. A. (2020). Self-Assembly of Minimal Peptoid Sequences. ACS Macro Letters, 2020(9), 494-499. https://doi.org/10.1021/acsmacrolett.9b01010