Segmental isotopic labelling of a multidomain protein by protein ligation by protein trans-splicing

Mikko Muona*, Sesilja Aranko, Hideo Iwai

*Corresponding author for this work

Research output: Contribution to journalArticleScientificpeer-review

39 Citations (Scopus)

Abstract

Structure–function relationships of intact multidomain proteins: Segmental isotopic labelling of multidomain proteins could open a new avenue for studying particular domains in intact proteins, because it could simplify the NMR spectra through the incorporation of stable isotopes in a region-specific manner. We have demonstrated that the necessary protein ligation can be easily achieved both in vivo and in vitro by protein trans-splicing.
Original languageEnglish
Pages (from-to)2958-2961
Number of pages4
JournalChemBioChem: a European journal of chemical biology
Volume9
Issue number18
DOIs
Publication statusPublished - 15 Dec 2008
MoE publication typeA1 Journal article-refereed

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