Structure–function relationships of intact multidomain proteins: Segmental isotopic labelling of multidomain proteins could open a new avenue for studying particular domains in intact proteins, because it could simplify the NMR spectra through the incorporation of stable isotopes in a region-specific manner. We have demonstrated that the necessary protein ligation can be easily achieved both in vivo and in vitro by protein trans-splicing.
|Number of pages||4|
|Journal||ChemBioChem: a European journal of chemical biology|
|Publication status||Published - 15 Dec 2008|
|MoE publication type||A1 Journal article-refereed|