Segmental isotopic labeling of a central domain in a multidomain protein by protein traws-splicing using only one robust dnae intein

Alena E L Busche; Sesilja Aranko; Mehdi Talebzadeh-Farooji; Frank Bernhard; Volker Dötsch; Hideo Iwaï

doi:10.1002/anie.200901488

Segmental isotopic labeling of a central domain in a multidomain protein by protein traws-splicing using only one robust dnae intein

Alena E L Busche, Sesilja Aranko, Mehdi Talebzadeh-Farooji, Frank Bernhard, Volker Dötsch, Hideo Iwaï^*

^*Corresponding author for this work

Not published at Aalto University

Research output: Contribution to journal › Article › Scientific › peer-review

54 Citations (Scopus)

Abstract

A split intein saves nine: A naturally split intein is used to create an efficient three-way ligation method that does not require any refolding steps. This method enables the selective labeling of a central domain within a three-domain protein, with NMR active isotopes (see picture) allowing domain-domain interactions in multidomain proteins to be investigated.

Original language	English
Pages (from-to)	6128-6131
Number of pages	4
Journal	Angewandte Chemie
Volume	48
Issue number	33
DOIs	https://doi.org/10.1002/anie.200901488
Publication status	Published - 2009
MoE publication type	A1 Journal article-refereed

Keywords

Inteins
Isotopic labeling
Nmr spectroscopy
Protein modifications
Protein splicing

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Segmental isotopic labeling of a central domain in a multidomain protein by protein traws-splicing using only one robust dnae intein

Abstract

Keywords

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