Segmental isotopic labeling of a central domain in a multidomain protein by protein traws-splicing using only one robust dnae intein

Alena E L Busche, Sesilja Aranko, Mehdi Talebzadeh-Farooji, Frank Bernhard, Volker Dötsch, Hideo Iwaï*

*Corresponding author for this work

Research output: Contribution to journalArticleScientificpeer-review

50 Citations (Scopus)

Abstract

A split intein saves nine: A naturally split intein is used to create an efficient three-way ligation method that does not require any refolding steps. This method enables the selective labeling of a central domain within a three-domain protein, with NMR active isotopes (see picture) allowing domain-domain interactions in multidomain proteins to be investigated.

Original languageEnglish
Pages (from-to)6128-6131
Number of pages4
JournalAngewandte Chemie
Volume48
Issue number33
DOIs
Publication statusPublished - 2009
MoE publication typeA1 Journal article-refereed

Keywords

  • Inteins
  • Isotopic labeling
  • Nmr spectroscopy
  • Protein modifications
  • Protein splicing

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