Scaling Up DNA Origami Lattice Assembly

Yang Xin, Boxuan Shen, Mauri A. Kostiainen, Guido Grundmeier, Mario Castro, Veikko Linko, Adrian Keller*

*Corresponding author for this work

Research output: Contribution to journalArticleScientificpeer-review

16 Citations (Scopus)
52 Downloads (Pure)


The surface-assisted hierarchical assembly of DNA origami nanostructures is a promising route to fabricate regular nanoscale lattices. In this work, the scalability of this approach is explored and the formation of a homogeneous polycrystalline DNA origami lattice at the mica-electrolyte interface over a total surface area of 18.75 cm2 is demonstrated. The topological analysis of more than 50 individual AFM images recorded at random locations over the sample surface showed only minuscule and random variations in the quality and order of the assembled lattice. The analysis of more than 450 fluorescence microscopy images of a quantum dot-decorated DNA origami lattice further revealed a very homogeneous surface coverage over cm2 areas with only minor boundary effects at the substrate edges. At total DNA costs of € 0.12 per cm2, this large-scale nanopatterning technique holds great promise for the fabrication of functional surfaces.

Original languageEnglish
Pages (from-to)8564-8571
Number of pages8
JournalChemistry - A European Journal
Issue number33
Early online date4 May 2021
Publication statusPublished - 10 Jun 2021
MoE publication typeA1 Journal article-refereed


  • DNA origami
  • lattice formation
  • molecular lithography
  • self-assembly
  • topological analysis


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