SACCHAROMYCES-CEREVISIAE CELLS SECRETING AN ASPERGILLUS-NIGER BETA-GALACTOSIDASE GROW ON WHEY PERMEATE

Pardeep KUMAR, S RAMAKRISHNAN, Tuula T. Teeri, JKC KNOWLES, BS HARTLEY

Research output: Contribution to journalArticleScientificpeer-review

Abstract

We describe the construction of a lactose-utilizing Saccharomyces cerevisiae that expresses the cDNA for a secreted, thermo-stable beta-galactosidase (lacA) from Aspergillus niger. Yeast cells expressing the lacA gene from the yeast ADH1 promoter on a multicopy plasmid secrete up to 40% of the total beta-galactosidase activity into the growth medium. The secreted product is extensively N-glycosylated, and cells expressing the lacA gene grow on whey permeate (4% w/v lactose) with a doubling time of 1.6 hours. Such strains may offer a solution to the increasing problem of waste whey disposal.

Original languageEnglish
Pages (from-to)82-85
Number of pages4
JournalBio-Technology
Volume10
Issue number1
Publication statusPublished - Jan 1992
MoE publication typeA1 Journal article-refereed

Keywords

  • YEAST
  • CONSTRUCTION
  • STRAINS
  • EXPRESSION
  • SEQUENCES
  • LACTOSE
  • CLONING
  • GENES

Cite this

KUMAR, P., RAMAKRISHNAN, S., Teeri, T. T., KNOWLES, JKC., & HARTLEY, BS. (1992). SACCHAROMYCES-CEREVISIAE CELLS SECRETING AN ASPERGILLUS-NIGER BETA-GALACTOSIDASE GROW ON WHEY PERMEATE. Bio-Technology, 10(1), 82-85.