Real-Time Observation of Superstructure-Dependent DNA Origami Digestion by DNase I Using High-Speed Atomic Force Microscopy

Saminathan Ramakrishnan, Boxuan Shen, Mauri A. Kostiainen, Guido Grundmeier, Adrian Keller*, Veikko Linko

*Corresponding author for this work

Research output: Contribution to journalArticleScientificpeer-review

19 Citations (Scopus)

Abstract

DNA nanostructures have emerged as intriguing tools for numerous biomedical applications. However, in many of those applications and most notably in drug delivery, their stability and function may be compromised by the biological media. A particularly important issue for medical applications is their interaction with proteins such as endonucleases, which may degrade the well-defined nanoscale shapes. Herein, fundamental insights into this interaction are provided by monitoring DNase I digestion of four structurally distinct DNA origami nanostructures (DONs) in real time and at a single-structure level by using high-speed atomic force microscopy. The effect of the solid–liquid interface on DON digestion is also assessed by comparison with experiments in bulk solution. It is shown that DON digestion is strongly dependent on its superstructure and flexibility and on the local topology of the individual structure.

Original languageEnglish
Pages (from-to)2818-2823
Number of pages6
JournalCHEMBIOCHEM
Volume20
Issue number22
DOIs
Publication statusPublished - 18 Nov 2019
MoE publication typeA1 Journal article-refereed

Keywords

  • DNA
  • DNA origami
  • endonucleases
  • high-speed atomic force microscopy
  • nanotechnology

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