Reaction Platforms for Enzymatic Testing

Eveliina Jutila, Risto Koivunen, Roger Bollström, Tiina Sikanen, Patrick Gane

    Research output: Chapter in Book/Report/Conference proceedingConference contributionScientificpeer-review

    Abstract

    The development of highly porous coating structures for reaction platforms, especially for enzymatic testing, is the focus of work presented here. Four types of coatings are formulated combining a highly porous form of pharmaceutical grade functionalised calcium carbonate (FCC) with two types of micro-fibrillated cellulose as binder (MFC A and MFC B), in which the fines content of MFC A and MFC B differ, MFC A having less freely dispersed film forming fines than MFC B. Additionally, two binder levels are adopted, namely 5 pph and 10 pph based on 100 pph by weight of FCC. Rod coating is used to meter the formulations onto an impermeable pigment filled polypropylene film. The coated substrate is then used as a base for hydrophobic patterning formed by printing each of two hydrophobic inks formulated based on polystyrene (PS) or alkyl ketene dimer (AKD), respectively. The study includes characterisation of the coating formulations, platform performance testing and design development at the stage prior to final application testing. The results show that the choice of binder
    as well as the binder amount has a significant effect on the porosity of the coatings, thus affecting the volume uptake and wetting capabilities of a test liquid in respect to a printed hydrophobic circular ring pattern. MFC A
    was seen to block/fill the inter-particle pores less than MFC B, though the volume uptake and evaporation times were greater for MFC B containing coatings, due to a slightly greater thickness of the coatings and the dependence of evaporation on diffusion and surface film flow rather than bulk flow. The results show inkjet-printing provides a convenient way to create patterns that can be easily adapted to suit the desired purposes depending on the functional properties of the coating. In future work, the suitability of the platforms will be tested using a model enzymatic reaction and ultimately developing a self-standing platform for enzymatic testing of pharmaceutical compounds.
    Original languageEnglish
    Title of host publicationAdvances in Printing and Media Technology
    Subtitle of host publicationToronto (2016)
    PublisherIARIGAI
    Pages39-49
    Volume43
    ISBN (Electronic) 978-3-9870704-1-9
    Publication statusPublished - 2016
    MoE publication typeA4 Article in a conference publication
    EventInternational Research Conference of Advances in Printing and Media Technology - Toronto, Canada
    Duration: 24 Aug 201627 Aug 2016
    Conference number: 43

    Publication series

    NameAdvances in Printing and Media Technology
    ISSN (Electronic)2409-4021

    Conference

    ConferenceInternational Research Conference of Advances in Printing and Media Technology
    Abbreviated titleIARIGAI
    CountryCanada
    CityToronto
    Period24/08/201627/08/2016

    Keywords

    • microfluidics
    • wicking
    • patterned coated paper
    • hydrophobic pattern
    • reaction platform

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