Rapid prototyping of tubular polyurethane and cell/hydrogel constructs

Kai He, Xiaohong Wang*

*Corresponding author for this work

    Research output: Contribution to journalArticleScientificpeer-review

    40 Citations (Scopus)

    Abstract

    A tubular polyurethane (PU) sandwich-like, adipose-derived stem cell (ADSC)/gelatin/alginate/ fibrin construct was fabricated using a double-nozzle, low-temperature (-20°C) deposition technique. The ADSCs survived the fabrication and cryopreservation stages by incorporating a cryoprotectant (glycerol or dimethyl sulfoxide (DMSO)) in the cell/hydrogel system. With 5% DMSO or 10% glycerol alone in the hydrogel, the cell viabilities were retained (73% and 62%, respectively). The three-dimensional construct was effectively preserved below -80°C for more than 1 week. After the freeze/thaw processes, cell viability and proliferation ability were regained. This strategy has the potential to be widely used in complex organ manufacturing techniques.

    Original languageEnglish
    Pages (from-to)363-374
    Number of pages12
    JournalJOURNAL OF BIOACTIVE AND COMPATIBLE POLYMERS
    Volume26
    Issue number4
    DOIs
    Publication statusPublished - Jul 2011
    MoE publication typeA1 Journal article-refereed

    Keywords

    • adipose-derived stem cells
    • double-nozzle
    • hydrogel
    • low-temperature cell loading
    • polyurethane
    • rapid prototyping
    • tissue regeneration

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