Radiolabeled Molecular Imaging Probes for the In Vivo Evaluation of Cellulose Nanocrystals for Biomedical Applications

Surachet Imlimthan, Sofia Otaru, Outi Keinänen, Alexandra Correia, Kalle Lintinen, Helder A. Santos, Anu J. Airaksinen, Mauri Kostiainen, Mirkka Sarparanta

Research output: Contribution to journalArticleScientificpeer-review

8 Citations (Scopus)
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Abstract

Cellulose nanocrystals (CNCs) have remarkable potential to improve the delivery of diagnostic and therapeutic agents to tumors; however, the in vivo studies on CNC biodistribution are still limited. We developed CNC-based imaging probes for the in vitro and in vivo evaluation using two labeling strategies: site-specific hydrazone linkage to the terminal aldehyde of the CNC and nonsite-specific activation using 1,1′-carbonyldiimidazole (CDI). The in vivo behavior of unmodified CNC, DOTA-CNC (ald.), and DOTA-CNC (OH) was investigated in healthy and 4T1 breast cancer mouse models. They displayed good biocompatibility in cell models. Moreover, the biodistribution profile and SPECT/CT imaging confirmed that the accumulation of 111 In-labeled DOTA-CNC (ald.) and 111 In-DOTA-CNC (OH) was primarily in hepatic, splenic, and pulmonary ducts in accordance with the clearance of nontargeted nanoparticles. The developed CNC imaging probes can be used to obtain information with noninvasive imaging on the behavior in vivo to guide structural optimization for targeted delivery.

Original languageEnglish
Pages (from-to)674-683
Number of pages10
JournalBiomacromolecules
Volume20
Issue number2
Early online date2018
DOIs
Publication statusPublished - 11 Feb 2019
MoE publication typeA1 Journal article-refereed

Keywords

  • DRUG-DELIVERY
  • SILICA NANOPARTICLES
  • SURFACE MODIFICATION
  • GOLD NANOPARTICLES
  • CANCER
  • CYTOTOXICITY
  • CHARGE
  • ANALOG
  • VITRO
  • SHAPE

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