Quantifying the interactions between biomimetic biomaterials – collagen I, collagen IV, laminin 521 and cellulose nanofibrils – by colloidal probe microscopy

Research output: Contribution to journalArticleScientificpeer-review

Researchers

Research units

  • University of Helsinki
  • Università Degli Studi di Padova

Abstract

Biomaterials of different nature have been and are widely studied for various biomedical applications. In many cases, biomaterial assemblies are designed to mimic biological systems. Although biomaterials have been thoroughly characterized in many aspects, not much quantitative information on the molecular level interactions between different biomaterials is available. That information is very important, on the one hand, to understand the properties of biological systems and, on the other hand, to develop new composite biomaterials for special applications. This work presents a systematic, quantitative analysis of self- and cross-interactions between films of collagen I (Col I), collagen IV (Col IV), laminin (LN-521), and cellulose nanofibrils (CNF), that is, biomaterials of different nature and structure that either exist in biological systems (e.g., extracellular matrices) or have shown potential for 3D cell culture and tissue engineering. Direct surface forces and adhesion between biomaterials-coated spherical microparticles and flat substrates were measured in phosphate-buffered saline using an atomic force microscope and the colloidal probe technique. Different methods (Langmuir-Schaefer deposition, spin-coating, or adsorption) were applied to completely coat the flat substrates and the spherical microparticles with homogeneous biomaterial films. The adhesion between biomaterials films increased with the time that the films were kept in contact. The strongest adhesion was observed between Col IV films, and between Col IV and LN-521 films after 30 s contact time. In contrast, low adhesion was measured between CNF films, as well as between CNF and LN-521 films. Nevertheless, a good adhesion between CNF and collagen films (especially Col I) was observed. These results increase our understanding of the structure of biological systems and can support the design of new matrices or scaffolds where different biomaterials are combined for diverse biological or medical applications.

Details

Original languageEnglish
Pages (from-to)571-580
Number of pages10
JournalColloids and Surfaces B: Biointerfaces
Volume173
Publication statusPublished - 1 Jan 2019
MoE publication typeA1 Journal article-refereed

    Research areas

  • Adhesion, AFM-colloidal probe technique, Cellulose nanofibrils, Collagen, Laminin, Surface forces, MODEL, PROTEOGLYCAN, MECHANICAL-PROPERTIES, ATOMIC-FORCE MICROSCOPE, HYDROGEL, MOLECULES, ORGANIZATION, FILMS, MICROENVIRONMENT, CELL

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