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Abstract
X-rays are widely used in mammography and radiotherapy of breast cancer. The research has focused on the effects of X-rays on cells in breast tissues, instead of the tissues' nonliving material, extracellular matrix. It is unclear what the influence of X-ray irradiation is on the matrix's mechanical cues, known to regulate malignant cancer-cell behaviors. Here, we developed a technique based on magnetic microrheology that can quantify the influence of X-ray irradiation on matrix viscoelasticity––or (solid-like) elastic and (liquid-like) viscous characteristics––at cell-size scales. To model breast-tissue extracellular matrix, we used the primary component of the tissue matrix, collagen type 1, as it is for control, and as irradiated by X-rays (tube voltage 50 kV). We used a magnetic microrheometer to measure collagen matrices using 10-μm-diameter magnetic probes. In each matrix, the probes were nanomanipulated using controlled magnetic forces by the microrheometer while the probes' displacements were detected to measure the viscoelasticity. The collagen-matrix data involve with a typical spatial variation in viscoelasticity. We find that higher irradiation doses (320 Gy) locally reduce stiffness (soften) collagen matrices and increase their loss tangent, indicating an elevated liquid-like nature. For lower, clinically relevant irradiation doses (54 Gy), we find insignificant matrix-viscoelasticity changes. We provide this irradiation-related technique for detection, and modification, of matrix viscoelastic cues at cell-size scales. The technique enables enhanced characterization of irradiated tissue constituents in a variety of breast-cancer radiotherapy types.
Original language | English |
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Article number | 044501 |
Number of pages | 9 |
Journal | Journal of biomechanical engineering : transactions of the ASME |
Volume | 146 |
Issue number | 4 |
Early online date | 29 Jan 2024 |
DOIs | |
Publication status | Published - Apr 2024 |
MoE publication type | A1 Journal article-refereed |
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Dive into the research topics of 'Quantifying the Influence of X-Ray Irradiation on Cell-Size-Scale Viscoelasticity of Collagen Type 1'. Together they form a unique fingerprint.Projects
- 1 Finished
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R2B-IMMUNATE: Personalize immunotherapies in breast cancer
Kyyrä, J. (Principal investigator), Arasalo, O. (Project Member), Aryal, U. (Project Member), Lanens, S. (Project Member), Lehtonen, A. (Project Member), Kielosto, M. (Project Member), Mäntylä, V. (Project Member) & Srbova, L. (Project Member)
01/01/2021 → 31/12/2022
Project: Business Finland: New business from research ideas (TUTLI)
Press/Media
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Study Findings from Aalto University Broaden Understanding of Cancer (Quantifying the Influence of X-Ray Irradiation On Cell-Size-scale Viscoelasticity of Collagen Type 1)
Pokki, J.
23/01/2024
1 item of Media coverage
Press/Media: Media appearance