Purification, crystallization and preliminary crystallographic analysis of the SpaA backbone-pilin subunit from probiotic Lactobacillus rhamnosus GG

Deepak Singh, Ingemar von Ossowski, Airi Palva, Vengadesan Krishnan*

*Corresponding author for this work

Research output: Contribution to journalArticleScientificpeer-review

Abstract

Lactobacillus rhamnosus GG, a widely used Gram-positive probiotic strain, is clinically well known for its perceived health-promoting effects. It has recently been shown to display proteinaceous pilus fibres (called SpaCBA) on its cell surface. Structurally, SpaCBA pili possess a characteristic three-pilin polymerized architecture, with repeating SpaA major pilins that form the backbone and two types of minor subunits (SpaB and SpaC). In this study, recombinant SpaA protein was purified, characterized and crystallized. The crystals diffracted to a resolution of 2.0 angstrom and belonged to space group C2, with unit-cell parameters a = 227.9, b = 63.2, c = 104.3 angstrom, beta = 95.1 degrees.

Original languageEnglish
Pages (from-to)1182-1185
Number of pages4
JournalActa crystallographica section F : Structural biology communications
Volume69
DOIs
Publication statusPublished - Oct 2013
MoE publication typeA1 Journal article-refereed

Keywords

  • STRUCTURAL BIOLOGY
  • ISOPEPTIDE BONDS
  • BACTERIAL
  • PROTEIN

Cite this