Lactobacillus rhamnosus GG, a widely used Gram-positive probiotic strain, is clinically well known for its perceived health-promoting effects. It has recently been shown to display proteinaceous pilus fibres (called SpaCBA) on its cell surface. Structurally, SpaCBA pili possess a characteristic three-pilin polymerized architecture, with repeating SpaA major pilins that form the backbone and two types of minor subunits (SpaB and SpaC). In this study, recombinant SpaA protein was purified, characterized and crystallized. The crystals diffracted to a resolution of 2.0 angstrom and belonged to space group C2, with unit-cell parameters a = 227.9, b = 63.2, c = 104.3 angstrom, beta = 95.1 degrees.
|Number of pages||4|
|Journal||Acta crystallographica section F : Structural biology communications|
|Publication status||Published - Oct 2013|
|MoE publication type||A1 Journal article-refereed|
- STRUCTURAL BIOLOGY
- ISOPEPTIDE BONDS