Abstract
The present article reports on the development of an effective downstream technique for the recovery of poly-ε-lysine (ε-PL), an unusual cationic homo-polyamide of L-lysine produced by fermentation using Streptomyces noursei NRRL 5126. Purification of ε-PL using cation exchange chromatography, ultrafiltration, solvent precipitation, and gel permeation chromatography was investigated. Loading of fermentation broth on Amberlite IRC 50 chromatographic column could purify the ε-PL with 95.84% recovery. Subsequent fractionation with ultrafiltration (20kDa, and 5kDa) was found to be effective at minimal transmembrane pressure and optimal flux which resulted in 91.66% product purity. Further, solvent precipitation and gel permeation chromatography which gave the highest possible purity (97.58%) and ε-PL yield (90.42%). Exclusion of ultrafiltration step negotiated the yield (88.62%) and purity (95.15) of ε-PL, if final application doesn't demand highly pure sample. Characterization with respect to molecular weight, thin layer chromatography, IR and NMR confirmed the identity of ε-PL in purified sample.
Original language | English |
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Pages (from-to) | 33-40 |
Number of pages | 8 |
Journal | Journal of Scientific and Industrial Research |
Volume | 73 |
Issue number | 1 |
Publication status | Published - Jan 2014 |
MoE publication type | A1 Journal article-refereed |
Keywords
- Characterization
- Gel filtration
- Poly-ε-lysine
- Purification
- Ultrafiltration
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