Purification and characterization of poly-ε-lysine from Streptomyces noursei NRRL 5126

Sandip B. Bankar, Sandeep A. Chaudhary, Rekha S. Singhal

Research output: Contribution to journalArticleScientificpeer-review

9 Citations (Scopus)

Abstract

The present article reports on the development of an effective downstream technique for the recovery of poly-ε-lysine (ε-PL), an unusual cationic homo-polyamide of L-lysine produced by fermentation using Streptomyces noursei NRRL 5126. Purification of ε-PL using cation exchange chromatography, ultrafiltration, solvent precipitation, and gel permeation chromatography was investigated. Loading of fermentation broth on Amberlite IRC 50 chromatographic column could purify the ε-PL with 95.84% recovery. Subsequent fractionation with ultrafiltration (20kDa, and 5kDa) was found to be effective at minimal transmembrane pressure and optimal flux which resulted in 91.66% product purity. Further, solvent precipitation and gel permeation chromatography which gave the highest possible purity (97.58%) and ε-PL yield (90.42%). Exclusion of ultrafiltration step negotiated the yield (88.62%) and purity (95.15) of ε-PL, if final application doesn't demand highly pure sample. Characterization with respect to molecular weight, thin layer chromatography, IR and NMR confirmed the identity of ε-PL in purified sample.

Original languageEnglish
Pages (from-to)33-40
Number of pages8
JournalJournal of Scientific and Industrial Research
Volume73
Issue number1
Publication statusPublished - Jan 2014
MoE publication typeA1 Journal article-refereed

Keywords

  • Characterization
  • Gel filtration
  • Poly-ε-lysine
  • Purification
  • Ultrafiltration

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