Proteome analysis of recombinant xylose-fermenting Saccharomyces cerevisiae

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Proteome analysis of recombinant xylose-fermenting Saccharomyces cerevisiae. / Salusjärvi, L; Poutanen, Marjo; Pitkänen, Juha-Pekka; Koivistoinen, H; Aristidou, Aristos; Kalkkinen, Nisse; Ruohonen, Laura; Penttilä, Merja.

In: Yeast, Vol. 20, No. 4, 03.2003, p. 295-314.

Research output: Contribution to journalArticle

Harvard

Salusjärvi, L, Poutanen, M, Pitkänen, J-P, Koivistoinen, H, Aristidou, A, Kalkkinen, N, Ruohonen, L & Penttilä, M 2003, 'Proteome analysis of recombinant xylose-fermenting Saccharomyces cerevisiae', Yeast, vol. 20, no. 4, pp. 295-314. https://doi.org/10.1002/yea.960

APA

Salusjärvi, L., Poutanen, M., Pitkänen, J-P., Koivistoinen, H., Aristidou, A., Kalkkinen, N., ... Penttilä, M. (2003). Proteome analysis of recombinant xylose-fermenting Saccharomyces cerevisiae. Yeast, 20(4), 295-314. https://doi.org/10.1002/yea.960

Vancouver

Salusjärvi L, Poutanen M, Pitkänen J-P, Koivistoinen H, Aristidou A, Kalkkinen N et al. Proteome analysis of recombinant xylose-fermenting Saccharomyces cerevisiae. Yeast. 2003 Mar;20(4):295-314. https://doi.org/10.1002/yea.960

Author

Salusjärvi, L ; Poutanen, Marjo ; Pitkänen, Juha-Pekka ; Koivistoinen, H ; Aristidou, Aristos ; Kalkkinen, Nisse ; Ruohonen, Laura ; Penttilä, Merja. / Proteome analysis of recombinant xylose-fermenting Saccharomyces cerevisiae. In: Yeast. 2003 ; Vol. 20, No. 4. pp. 295-314.

Bibtex - Download

@article{7bf07064d7d2415b96a2cd42256ab55c,
title = "Proteome analysis of recombinant xylose-fermenting Saccharomyces cerevisiae",
abstract = "Introduction of an active xylose utilization pathway into Saccharomyces cerevisiae, which does not naturally ferment pentose sugars, is likely to have a major impact on the overall cellular metabolism as the carbon introduced to the cells will now flow through the pentose phosphate pathway. The metabolic responses in the recombinant, xylose-fermenting S. cerevisiae were studied at the proteome level by comparative two-dimensional gel electrophoresis of cellular proteins within a pH range of 3-10. Glucose-limited chemostat cultivations and corresponding chemostat cultivations performed in media containing xylose as the major carbon source were compared. The cultivations were studied in aerobic and anaerobic metabolic steady states and in addition at time points 5, 30 and 60 min after the switch-off of oxygen supply. We identified 22 proteins having a significant abundance difference on xylose compared to glucose, and 12 proteins that responded to change from aerobic to anaerobic conditions on both carbon sources. On xylose in all conditions studied, major changes were seen in the abundance of alcohol dehydrogenase 2 (Adh2p), acetaldehyde dehydrogenases 4 and 6 (Ald4p and Ald6p), and DL-glycerol 3-phosphatase (Gpp1p). Our results give indications of altered metabolic fluxes especially in the acetate and glycerol pathways in cells growing on xylose compared to glucose. Copyright (C) 2003 John Wiley Sons, Ltd.",
keywords = "Saccharomyces cerevisiae, xylose, ethanol, proteome, proteomics, two-dimensional gel electrophoresis, pentose phosphate pathway, metabolic engineering, 2-DIMENSIONAL GEL-ELECTROPHORESIS, GLUCOSE-FRUCTOSE OXIDOREDUCTASE, PYRUVATE-DEHYDROGENASE BYPASS, MASS-SPECTROMETRY, GENE-EXPRESSION, ACETALDEHYDE DEHYDROGENASES, XYLITOL DEHYDROGENASE, CHEMOSTAT CULTURES, ZYMOMONAS-MOBILIS, ESCHERICHIA-COLI",
author = "L Salusj{\"a}rvi and Marjo Poutanen and Juha-Pekka Pitk{\"a}nen and H Koivistoinen and Aristos Aristidou and Nisse Kalkkinen and Laura Ruohonen and Merja Penttil{\"a}",
year = "2003",
month = "3",
doi = "10.1002/yea.960",
language = "English",
volume = "20",
pages = "295--314",
journal = "Yeast",
issn = "0749-503X",
number = "4",

}

RIS - Download

TY - JOUR

T1 - Proteome analysis of recombinant xylose-fermenting Saccharomyces cerevisiae

AU - Salusjärvi, L

AU - Poutanen, Marjo

AU - Pitkänen, Juha-Pekka

AU - Koivistoinen, H

AU - Aristidou, Aristos

AU - Kalkkinen, Nisse

AU - Ruohonen, Laura

AU - Penttilä, Merja

PY - 2003/3

Y1 - 2003/3

N2 - Introduction of an active xylose utilization pathway into Saccharomyces cerevisiae, which does not naturally ferment pentose sugars, is likely to have a major impact on the overall cellular metabolism as the carbon introduced to the cells will now flow through the pentose phosphate pathway. The metabolic responses in the recombinant, xylose-fermenting S. cerevisiae were studied at the proteome level by comparative two-dimensional gel electrophoresis of cellular proteins within a pH range of 3-10. Glucose-limited chemostat cultivations and corresponding chemostat cultivations performed in media containing xylose as the major carbon source were compared. The cultivations were studied in aerobic and anaerobic metabolic steady states and in addition at time points 5, 30 and 60 min after the switch-off of oxygen supply. We identified 22 proteins having a significant abundance difference on xylose compared to glucose, and 12 proteins that responded to change from aerobic to anaerobic conditions on both carbon sources. On xylose in all conditions studied, major changes were seen in the abundance of alcohol dehydrogenase 2 (Adh2p), acetaldehyde dehydrogenases 4 and 6 (Ald4p and Ald6p), and DL-glycerol 3-phosphatase (Gpp1p). Our results give indications of altered metabolic fluxes especially in the acetate and glycerol pathways in cells growing on xylose compared to glucose. Copyright (C) 2003 John Wiley Sons, Ltd.

AB - Introduction of an active xylose utilization pathway into Saccharomyces cerevisiae, which does not naturally ferment pentose sugars, is likely to have a major impact on the overall cellular metabolism as the carbon introduced to the cells will now flow through the pentose phosphate pathway. The metabolic responses in the recombinant, xylose-fermenting S. cerevisiae were studied at the proteome level by comparative two-dimensional gel electrophoresis of cellular proteins within a pH range of 3-10. Glucose-limited chemostat cultivations and corresponding chemostat cultivations performed in media containing xylose as the major carbon source were compared. The cultivations were studied in aerobic and anaerobic metabolic steady states and in addition at time points 5, 30 and 60 min after the switch-off of oxygen supply. We identified 22 proteins having a significant abundance difference on xylose compared to glucose, and 12 proteins that responded to change from aerobic to anaerobic conditions on both carbon sources. On xylose in all conditions studied, major changes were seen in the abundance of alcohol dehydrogenase 2 (Adh2p), acetaldehyde dehydrogenases 4 and 6 (Ald4p and Ald6p), and DL-glycerol 3-phosphatase (Gpp1p). Our results give indications of altered metabolic fluxes especially in the acetate and glycerol pathways in cells growing on xylose compared to glucose. Copyright (C) 2003 John Wiley Sons, Ltd.

KW - Saccharomyces cerevisiae

KW - xylose

KW - ethanol

KW - proteome

KW - proteomics

KW - two-dimensional gel electrophoresis

KW - pentose phosphate pathway

KW - metabolic engineering

KW - 2-DIMENSIONAL GEL-ELECTROPHORESIS

KW - GLUCOSE-FRUCTOSE OXIDOREDUCTASE

KW - PYRUVATE-DEHYDROGENASE BYPASS

KW - MASS-SPECTROMETRY

KW - GENE-EXPRESSION

KW - ACETALDEHYDE DEHYDROGENASES

KW - XYLITOL DEHYDROGENASE

KW - CHEMOSTAT CULTURES

KW - ZYMOMONAS-MOBILIS

KW - ESCHERICHIA-COLI

U2 - 10.1002/yea.960

DO - 10.1002/yea.960

M3 - Article

VL - 20

SP - 295

EP - 314

JO - Yeast

JF - Yeast

SN - 0749-503X

IS - 4

ER -

ID: 2403865