Protein trans-splicing as a protein ligation tool to study protein structure and function

Sesilja Aranko, Gerrit Volkmanna*

*Corresponding author for this work

Research output: Contribution to journalReview Articlepeer-review

8 Citations (Scopus)


Protein trans-splicing (PTS) exerted by split inteins is a protein ligation reaction which enables overcoming the barriers of conventional heterologous protein production. We provide an overview of the current state-of-the-art in split intein engineering, as well as the achievements of PTS technology in the realm of protein structure-function analyses, including incorporation of natural and artificial protein modifications, controllable protein reconstitution, segmental isotope labeling and protein cyclization. We further discuss factors crucial for the successful implementation of PTS in these protein engineering approaches, and speculate on necessary future endeavours to make PTS a universally applicable protein ligation tool.

Original languageEnglish
Pages (from-to)183-198
Number of pages16
Issue number3
Publication statusPublished - 1 Jun 2011
MoE publication typeA2 Review article in a scientific journal


  • junction sequence effect
  • protein ligation
  • protein semisynthesis
  • protein structure-function relationships
  • protein trans-splicing
  • split intein


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