Protein dot stamp using hydrophobin as immobilization carrier

Shinya Ikeno, Géza R. Szilvay, Markus Linder, Hijiri Aritomi, Masako Ajimi, Tetsuya Haruyama*

*Corresponding author for this work

Research output: Contribution to journalArticleScientificpeer-review

8 Citations (Scopus)

Abstract

Protein dot spot technology is presented using unique proteins such as hydrophobin HFB (from Trichoderma reesei). HFB is a protein of low molecular weight (7.8 kD) and is an outstanding hydrophobic molecule. The authors employ HFB as a carrier for protein dot spots. In this study, fluorescent-labeled HFB was spotted on a hydrophobic glass substrate using a solution drop contact spotter. HFB adsorbed on the glass surface uniformly. The results show that HFB provides prominent adsorption characteristics as a carrier protein. The small HFB molecule can be tagged onto other protein molecules through a conventional genetic procedure. By using HFB as a carrier protein, practical protein chip technology can be realized.

Original languageEnglish
Pages (from-to)413-420
Number of pages8
JournalSensors and Materials
Volume16
Issue number8
Publication statusPublished - 2004
MoE publication typeA1 Journal article-refereed

Keywords

  • Dot spot
  • High throughput
  • Hydrophobin
  • Protein array

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    Ikeno, S., Szilvay, G. R., Linder, M., Aritomi, H., Ajimi, M., & Haruyama, T. (2004). Protein dot stamp using hydrophobin as immobilization carrier. Sensors and Materials, 16(8), 413-420.