Protein Adsorption and Coordination-Based End-Tethering of Functional Polymers on Metal-Phenolic Network Films

Research output: Contribution to journalArticleScientificpeer-review

Researchers

  • Blaise Tardy

  • Joseph J. Richardson
  • Vichida Nithipipat
  • Kristian Kempe
  • Junling Guo
  • Kwun Lun Cho
  • Md Arifur Rahim
  • Hirotaka Ejima
  • Frank Caruso

Research units

  • Monash University
  • Sichuan University
  • University of Tokyo
  • University of Melbourne

Abstract

Metal-phenolic network (MPN) coatings have generated increasing interest owing to their biologically inspired nature, facile fabrication, and near-universal adherence, especially for biomedical applications. However, a key issue in biomedicine is protein fouling, and the adsorption of proteins on tannic acid-based MPNs remains to be comprehensively studied. Herein, we investigate the interaction of specific biomedically relevant proteins in solution (e.g., bovine serum albumin (BSA), immunoglobulin G (IgG), fibrinogen) and complex biological media (serum) using layer-by-layer-assembled tannic acid/Fe III MPN films. When Fe III was the outermost layer, galloyl-modified poly(2-ethyl-2-oxazoline) (P(EtOx)-Gal) could be grafted to the films through coordination bonds. Protein fouling and bacterial adhesion were greatly suppressed after functionalization with P(EtOx)-Gal and the mass of adsorbed protein was reduced by 79%. Interestingly, larger proteins adsorbed more on both the MPNs and P(EtOx)-functionalized MPNs. This study provides fundamental information on the interactions of MPNs with single proteins, mixtures of proteins as encountered in serum, and the noncovalent, coordination-based, functionalization of MPN films.

Details

Original languageEnglish
Pages (from-to)1421-1428
Number of pages8
JournalBiomacromolecules
Volume20
Issue number3
Publication statusPublished - 11 Mar 2019
MoE publication typeA1 Journal article-refereed

ID: 32664390