Production of poly-β-hydroxybutyrate by Alcaligenes eutrophus on different carbon sources

Susan Linko*, Hanna Vaheri, Jukka Seppälä

*Corresponding author for this work

    Research output: Contribution to journalArticleScientificpeer-review

    16 Citations (Scopus)


    Poly-β-hydroxybutyrate was produced in shake cultures by Alcaligenes eutrophus H16 on fructose, xylose, and fumaric, itaconic, lactic and propionic acids in a three-stage process. The maximum polymer concentration of 6.9 g l-1 (69% of cell dry matter) was obtained with 20g l-1 of fructose with a volumetric productivity of about 0.22 g l-1 h-1 at 24h. Up to about 3 g l-1 (about 50% of cell dry matter) of polymer was also produced on lactic and propionic acids as the sole carbon source during the production phase. In multivatiate optimization employing an orthogonal 23-factorial central composite experimental design with fructose as the substrate in a single-stage process, the optimal initial fructose concentration decreased from 35 g l-1 to 24 g l-1 when the incubation time was increased from about 35 h to 96 h. The optimal shaking speed range was 90-113 rpm.

    Original languageEnglish
    Pages (from-to)11-15
    Number of pages5
    JournalApplied Microbiology and Biotechnology
    Issue number1
    Publication statusPublished - Apr 1993
    MoE publication typeA1 Journal article-refereed


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