Abstract
The fungal pathway for L-arabinose catabolism converts c-arabinose to D-xylulose 5-phosphate in five steps. The intermediates are, in this order: L-arabinitol, L-xylulose, xylitol and D-xylulose. Only some of the genes for the corresponding enzymes were known. We have recently identified the two missing genes for L-arabinitol 4-dehydrogenase and L-xylulose reductase and shown that overexpression of all the genes of the pathway in Saccharomyces cerevisiae enables growth on L-arabinose. Under anaerobic conditions ethanol is produced from L-arabinose, but at a very low rate. The reasons for the low rate of L-arabinose fermentation are discussed. (C) 2002 Federation of European Microbiological Societies. Published by Elsevier Science B.V. All rights reserved.
| Original language | English |
|---|---|
| Pages (from-to) | 185-189 |
| Number of pages | 5 |
| Journal | FEMS Yeast Research |
| Volume | 3 |
| Issue number | 2 |
| DOIs | |
| Publication status | Published - Apr 2003 |
| MoE publication type | A1 Journal article-refereed |
| Event | International Specialized Symposium on Yeasts - Sun City, South Africa Duration: 25 Mar 2002 → 28 Mar 2002 Conference number: 22 |
Keywords
- L-arabinose
- pentose fermentation
- L-arabinitol 4-dehydrogenase
- L-xylulose reductase
- Saccharomyces cerevisiae
- XYLOSE ISOMERASE GENE
- ESCHERICHIA-COLI
- XYLITOL DEHYDROGENASE
- PICHIA-STIPITIS
- EXPRESSION
- CLONING
- GLUCOSE
- YEAST
- XYLULOKINASE
- FERMENTATION