Production of biopolymer precursors beta-alanine and L-lactic acid from CO₂ with metabolically versatile Rhodococcus opacus DSM 43205

Hydrogen oxidizing autotrophic bacteria are promising hosts for conversion of CO₂ into chemicals. In this work, we engineered the metabolically versatile lithoautotrophic bacterium R. opacus strain DSM 43205 for synthesis of polymer precursors. Aspartate decarboxylase (panD) or lactate dehydrogenase (ldh) were expressed for beta-alanine or L-lactic acid production, respectively. The heterotrophic cultivations on glucose produced 25 mg L⁻¹ beta-alanine and 742 mg L⁻¹ L-lactic acid, while autotrophic cultivations with CO₂, H₂, and O₂ resulted in the production of 1.8 mg L⁻¹ beta-alanine and 146 mg L⁻¹ L-lactic acid. Beta-alanine was also produced at 345 μg L⁻¹ from CO₂ in electrobioreactors, where H₂ and O₂ were provided by water electrolysis. This work demonstrates that R. opacus DSM 43205 can be engineered to produce chemicals from CO₂ and provides a base for its further metabolic engineering.