Process technology for production and recovery of heterologous proteins with Pichia pastoris

Mehmedalija Jahic*, Andres Veide, Theppanya Charoenrat, Tuula Teeri, Sven-Olof Enfors

*Corresponding author for this work

Research output: Contribution to journalLiterature reviewScientificpeer-review

Abstract

Developments in process techniques for production and recovery of heterologous proteins with Pichia pastoris are presented. Limitations for the standard techniques are described, and alternative techniques that solve the limitations problems are reviewed together with the methods that resulted in higher productivity of the P. pastoris processes. The main limitations are proteolysis of the secreted products and cell death in the high cell density bioreactor cultures. As a consequence, both low productivity and lower quality of the feedstock for downstream processing are achieved in processes hampered with these problems. Methods for exploring proteolysis and cell death are also presented. Solving the problems makes the conditions for downstream processing superior for the P. pastoris expression systems compared to other systems, which either need complex media or rely on intracellular production. These improved conditions allow for interfacing of cultivation with downstream processing in an integrated fashion.

Original languageEnglish
Pages (from-to)1465-1473
Number of pages9
JournalBiotechnology Progress
Volume22
Issue number6
DOIs
Publication statusPublished - 1 Dec 2006
MoE publication typeA1 Journal article-refereed

Keywords

  • EXPANDED-BED ADSORPTION
  • FED-BATCH FERMENTATION
  • HIGH-LEVEL PRODUCTION
  • HUMAN SERUM-ALBUMIN
  • RECOMBINANT PROTEIN
  • METHYLOTROPHIC YEAST
  • SACCHAROMYCES-CEREVISIAE
  • METHANOL CONCENTRATION
  • ESCHERICHIA-COLI
  • FEEDING STRATEGY

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