Preparation and characterization of platinum coatings for long life-time BOD biosensor

K. Tammeveski, T. Kikas, T. Tenno, L. Niinistö

    Research output: Contribution to journalArticleScientificpeer-review

    Abstract

    An easy method to prepare platinum coatings is proposed. Platinum coatings were prepared on glass substrate by applying a thermal decomposition method. Electrochemical and surface properties of the Pt coatings obtained were studied. A polycrystalline Pt layer is formed which shows electrocatalytic activity towards oxygen reduction similar to that of bulk platinum. An amperometric oxygen sensor was constructed by using Pt-coated glass substrate as a cathode. The oxygen sensor exhibited good performance characteristics. A biosensor for the determination of biochemical oxygen demand (BOD) was developed using the oxygen sensor as a base transducer and by immobilizing bacteria Bacillus subtilis in agarose gel film on the top of the sensor's membrane. The BOD biosensor was tested in various media.
    Original languageEnglish
    Pages (from-to)21-29
    JournalSensors and Actuators B: Chemical
    VolumeB47
    DOIs
    Publication statusPublished - 1998
    MoE publication typeA1 Journal article-refereed

    Keywords

    • biosensor
    • BOD
    • platinum

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