Potato virus A particles – A versatile material for self-assembled nanopatterned surfaces

Swarnalok De; Hoang M. Nguyen; Ville Liljeström; Kristiina Mäkinen; Mauri Kostiainen; Jaana Vapaavuori

doi:10.1016/j.virol.2022.11.010

Potato virus A particles – A versatile material for self-assembled nanopatterned surfaces

Swarnalok De, Hoang M. Nguyen, Ville Liljeström, Kristiina Mäkinen, Mauri Kostiainen^*, Jaana Vapaavuori^*

^*Corresponding author for this work

University of Helsinki

Research output: Contribution to journal › Article › Scientific › peer-review

3 Citations (Scopus)

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Abstract

Potato virus A (PVA) is a plant-infecting RNA virus that produces flexible particles with a high aspect ratio. PVA has been investigated extensively for its infection biology, however, its potential to serve as a nanopatterning platform remains unexplored. Here, we study the liquid crystal and interfacial self-assembly behavior of PVA particles. Furthermore, we generate nanopatterned surfaces using self-assembled PVA particles through three different coating techniques: drop-casting, drop-top deposition and flow-coating. The liquid crystal phase of PVA solution visualized by polarized optical microscopy revealed a chiral nematic phase in water, while in pH 8 buffer it produced a nematic phase. This allowed us to produce thin films with either randomly or anisotropically oriented cylindrical nanopatterns using drop-top and flow-coating methods. Overall, this study explores the self-assembly process of PVA in different conditions, establishing a starting point for PVA self-assembly research and contributing a virus-assisted fabrication technique for nanopatterned surfaces.

Original language	English
Pages (from-to)	103-110
Journal	Virology
Volume	578
Early online date	6 Dec 2022
DOIs	https://doi.org/10.1016/j.virol.2022.11.010
Publication status	Published - Jan 2023
MoE publication type	A1 Journal article-refereed

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10.1016/j.virol.2022.11.010Licence: CC BY

CHEM_De_et_al_Potato_virus_A_particles_2022_VirologyFinal published version, 7.62 MBLicence: CC BY

Cite this

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title = "Potato virus A particles – A versatile material for self-assembled nanopatterned surfaces",

abstract = "Potato virus A (PVA) is a plant-infecting RNA virus that produces flexible particles with a high aspect ratio. PVA has been investigated extensively for its infection biology, however, its potential to serve as a nanopatterning platform remains unexplored. Here, we study the liquid crystal and interfacial self-assembly behavior of PVA particles. Furthermore, we generate nanopatterned surfaces using self-assembled PVA particles through three different coating techniques: drop-casting, drop-top deposition and flow-coating. The liquid crystal phase of PVA solution visualized by polarized optical microscopy revealed a chiral nematic phase in water, while in pH 8 buffer it produced a nematic phase. This allowed us to produce thin films with either randomly or anisotropically oriented cylindrical nanopatterns using drop-top and flow-coating methods. Overall, this study explores the self-assembly process of PVA in different conditions, establishing a starting point for PVA self-assembly research and contributing a virus-assisted fabrication technique for nanopatterned surfaces.",

author = "Swarnalok De and Nguyen, {Hoang M.} and Ville Liljestr{\"o}m and Kristiina M{\"a}kinen and Mauri Kostiainen and Jaana Vapaavuori",

note = "| openaire: EC/H2020/949648/EU//ModelCom The research in Multifunctional Material Design lab (Aalto University) was funded by The Ella Georg Ehrnrooth Foundation, the European Research Council (H2020) StG “Autonomously adapting and communicating modular textiles” No. 949648 ModelCom, Academy of Finland SUPER-WEAR project (decision number: 322214), ProCrystal (decision 680210), Academy of Finland's Flagship Programme under Projects No. 318890 and 318891 (Competence Center for Materials Bioeconomy, FinnCERES). Research in the M{\"a}kinen lab (University of Helsinki) is supported by Academy of Finland (decision 332950). Further, we acknowledge OtaNano Nanomicroscopy Center at Aalto University for their facilities and expertise that were crucial for the SAXS and TEM studies carried out in this work. We also Acknowledge HiLIFE Biocomplex unit at the University of Helsinki, a member of Instruct-ERIC Centre Finland, FINStruct, and Biocenter Finland for their facilities and expertise that were crucial for the purification of PVA VNPs.",

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doi = "10.1016/j.virol.2022.11.010",

language = "English",

volume = "578",

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T1 - Potato virus A particles – A versatile material for self-assembled nanopatterned surfaces

AU - De, Swarnalok

AU - Nguyen, Hoang M.

AU - Liljeström, Ville

AU - Mäkinen, Kristiina

AU - Kostiainen, Mauri

AU - Vapaavuori, Jaana

N1 - | openaire: EC/H2020/949648/EU//ModelCom The research in Multifunctional Material Design lab (Aalto University) was funded by The Ella Georg Ehrnrooth Foundation, the European Research Council (H2020) StG “Autonomously adapting and communicating modular textiles” No. 949648 ModelCom, Academy of Finland SUPER-WEAR project (decision number: 322214), ProCrystal (decision 680210), Academy of Finland's Flagship Programme under Projects No. 318890 and 318891 (Competence Center for Materials Bioeconomy, FinnCERES). Research in the Mäkinen lab (University of Helsinki) is supported by Academy of Finland (decision 332950). Further, we acknowledge OtaNano Nanomicroscopy Center at Aalto University for their facilities and expertise that were crucial for the SAXS and TEM studies carried out in this work. We also Acknowledge HiLIFE Biocomplex unit at the University of Helsinki, a member of Instruct-ERIC Centre Finland, FINStruct, and Biocenter Finland for their facilities and expertise that were crucial for the purification of PVA VNPs.

PY - 2023/1

Y1 - 2023/1

N2 - Potato virus A (PVA) is a plant-infecting RNA virus that produces flexible particles with a high aspect ratio. PVA has been investigated extensively for its infection biology, however, its potential to serve as a nanopatterning platform remains unexplored. Here, we study the liquid crystal and interfacial self-assembly behavior of PVA particles. Furthermore, we generate nanopatterned surfaces using self-assembled PVA particles through three different coating techniques: drop-casting, drop-top deposition and flow-coating. The liquid crystal phase of PVA solution visualized by polarized optical microscopy revealed a chiral nematic phase in water, while in pH 8 buffer it produced a nematic phase. This allowed us to produce thin films with either randomly or anisotropically oriented cylindrical nanopatterns using drop-top and flow-coating methods. Overall, this study explores the self-assembly process of PVA in different conditions, establishing a starting point for PVA self-assembly research and contributing a virus-assisted fabrication technique for nanopatterned surfaces.

AB - Potato virus A (PVA) is a plant-infecting RNA virus that produces flexible particles with a high aspect ratio. PVA has been investigated extensively for its infection biology, however, its potential to serve as a nanopatterning platform remains unexplored. Here, we study the liquid crystal and interfacial self-assembly behavior of PVA particles. Furthermore, we generate nanopatterned surfaces using self-assembled PVA particles through three different coating techniques: drop-casting, drop-top deposition and flow-coating. The liquid crystal phase of PVA solution visualized by polarized optical microscopy revealed a chiral nematic phase in water, while in pH 8 buffer it produced a nematic phase. This allowed us to produce thin films with either randomly or anisotropically oriented cylindrical nanopatterns using drop-top and flow-coating methods. Overall, this study explores the self-assembly process of PVA in different conditions, establishing a starting point for PVA self-assembly research and contributing a virus-assisted fabrication technique for nanopatterned surfaces.

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U2 - 10.1016/j.virol.2022.11.010

DO - 10.1016/j.virol.2022.11.010

M3 - Article

SN - 0042-6822

VL - 578

SP - 103

EP - 110

JO - Virology

JF - Virology

ER -

Potato virus A particles – A versatile material for self-assembled nanopatterned surfaces

Abstract

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ModelCom: Autonomously adapting and communicating modular textiles

-: Super-stretchable functionalized materials and fibers for third generation wearable technology

FinnCERES: Competence Center for the Materials Bioeconomy: A Flagship for our Sustainable Future

OtaNano

OtaNano - Nanomicroscopy Center

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Potato virus A particles – A versatile material for self-assembled nanopatterned surfaces

Abstract

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Projects

ModelCom: Autonomously adapting and communicating modular textiles

-: Super-stretchable functionalized materials and fibers for third generation wearable technology

FinnCERES: Competence Center for the Materials Bioeconomy: A Flagship for our Sustainable Future

Equipment

OtaNano

OtaNano - Nanomicroscopy Center

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