Piezoelectric Sensors to Screen Enzymes in Cellulose Processing

    Research output: Chapter in Book/Report/Conference proceedingConference contributionScientificpeer-review

    Abstract

    The use of the Quartz Crystal Microbalance (QCM) technique is discussed in terms of its capability to screen enzymatic activities in cellulose-related applications, in-situ and in real time. Two approaches are discussed: (1) The development and application of a biosensor based on various cellulose model films (including spin coated cellulose, cellulose nanocrystals and microfibrillar cellulose) and, (2) the use of an un-modified gold sensor to monitor and quantify the products of degradation upon substrate incubation. The variations registered in QCM frequency and energy dissipation were found to correlate with mass and morphological transformations due to enzyme activities. A simple empirical model that included kinetic parameters for binding and hydrolysis events was used to quantify and compare different enzymatic treatments (incubation conditions and enzyme formulations). The applicability of this technique in fiber processing and in biomass-related operations is highlighted and some reference cases discussed.

    Original languageEnglish
    Title of host publicationPROCEEDINGS OF INTERNATIONAL CONFERENCE ON PULPING, PAPERMAKING AND BIOTECHNOLOGY 2008: ICPPB '08, VOL I
    EditorsY Jin, H Zhai, Z Li
    Pages726-731
    Number of pages6
    Publication statusPublished - 2008
    MoE publication typeA4 Article in a conference publication
    EventInternational Conference on Pulping, Papermaking and Biotechnology - Nanjing
    Duration: 4 Nov 20086 Nov 2008

    Conference

    ConferenceInternational Conference on Pulping, Papermaking and Biotechnology
    CityNanjing
    Period04/11/200806/11/2008

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