Photoluminescent Hybrids of Cellulose Nanocrystals and Carbon Quantum Dots as Cytocompatible Probes for in Vitro Bioimaging

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Photoluminescent Hybrids of Cellulose Nanocrystals and Carbon Quantum Dots as Cytocompatible Probes for in Vitro Bioimaging. / Guo, Jiaqi; Liu, Dongfei; Filpponen, Ilari; Johansson, Leena Sisko; Malho, Jani Markus; Quraishi, Sakeena; Liebner, Falk; Santos, Hélder A.; Rojas, Orlando J.

In: Biomacromolecules, Vol. 18, No. 7, 10.07.2017, p. 2045-2055.

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Guo, Jiaqi ; Liu, Dongfei ; Filpponen, Ilari ; Johansson, Leena Sisko ; Malho, Jani Markus ; Quraishi, Sakeena ; Liebner, Falk ; Santos, Hélder A. ; Rojas, Orlando J. / Photoluminescent Hybrids of Cellulose Nanocrystals and Carbon Quantum Dots as Cytocompatible Probes for in Vitro Bioimaging. In: Biomacromolecules. 2017 ; Vol. 18, No. 7. pp. 2045-2055.

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@article{88cab1671f8b43f5b2d170271dd0eaba,
title = "Photoluminescent Hybrids of Cellulose Nanocrystals and Carbon Quantum Dots as Cytocompatible Probes for in Vitro Bioimaging",
abstract = "We present an approach to construct biocompatible and photoluminescent hybrid materials comprised of carbon quantum dots (CQDs) and TEMPO-oxidized cellulose nanocrystals (TO-CNCs). First, the amino-functionalized carbon quantum dots (NH2-CQDs) were synthesized using a simple microwave method, and the TO-CNCs were prepared by hydrochloric acid (HCl) hydrolysis followed by TEMPO-mediated oxidation. The conjugation of NH2-CQDs and TO-CNCs was conducted via carbodiimide-assisted coupling chemistry. The synthesized TO-CNC@CQD hybrid nanomaterials were characterized using X-ray photoelectron spectroscopy, cryo-transmittance electron microscopy, confocal microscopy, and fluorescence spectroscopy. Finally, the interactions of TO-CNC@CQD hybrids with HeLa and RAW 264.7 macrophage cells were investigated in vitro. Cell viability tests suggest the surface conjugation with NH2-CQDs not only improved the cytocompatibility of TO-CNCs, but also enhanced their cellular association and internalization on both HeLa and RAW 264.7 cells after 4 and 24 h incubation.",
author = "Jiaqi Guo and Dongfei Liu and Ilari Filpponen and Johansson, {Leena Sisko} and Malho, {Jani Markus} and Sakeena Quraishi and Falk Liebner and Santos, {H{\'e}lder A.} and Rojas, {Orlando J.}",
year = "2017",
month = "7",
day = "10",
doi = "10.1021/acs.biomac.7b00306",
language = "English",
volume = "18",
pages = "2045--2055",
journal = "Biomacromolecules",
issn = "1525-7797",
publisher = "AMERICAN CHEMICAL SOCIETY",
number = "7",

}

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TY - JOUR

T1 - Photoluminescent Hybrids of Cellulose Nanocrystals and Carbon Quantum Dots as Cytocompatible Probes for in Vitro Bioimaging

AU - Guo, Jiaqi

AU - Liu, Dongfei

AU - Filpponen, Ilari

AU - Johansson, Leena Sisko

AU - Malho, Jani Markus

AU - Quraishi, Sakeena

AU - Liebner, Falk

AU - Santos, Hélder A.

AU - Rojas, Orlando J.

PY - 2017/7/10

Y1 - 2017/7/10

N2 - We present an approach to construct biocompatible and photoluminescent hybrid materials comprised of carbon quantum dots (CQDs) and TEMPO-oxidized cellulose nanocrystals (TO-CNCs). First, the amino-functionalized carbon quantum dots (NH2-CQDs) were synthesized using a simple microwave method, and the TO-CNCs were prepared by hydrochloric acid (HCl) hydrolysis followed by TEMPO-mediated oxidation. The conjugation of NH2-CQDs and TO-CNCs was conducted via carbodiimide-assisted coupling chemistry. The synthesized TO-CNC@CQD hybrid nanomaterials were characterized using X-ray photoelectron spectroscopy, cryo-transmittance electron microscopy, confocal microscopy, and fluorescence spectroscopy. Finally, the interactions of TO-CNC@CQD hybrids with HeLa and RAW 264.7 macrophage cells were investigated in vitro. Cell viability tests suggest the surface conjugation with NH2-CQDs not only improved the cytocompatibility of TO-CNCs, but also enhanced their cellular association and internalization on both HeLa and RAW 264.7 cells after 4 and 24 h incubation.

AB - We present an approach to construct biocompatible and photoluminescent hybrid materials comprised of carbon quantum dots (CQDs) and TEMPO-oxidized cellulose nanocrystals (TO-CNCs). First, the amino-functionalized carbon quantum dots (NH2-CQDs) were synthesized using a simple microwave method, and the TO-CNCs were prepared by hydrochloric acid (HCl) hydrolysis followed by TEMPO-mediated oxidation. The conjugation of NH2-CQDs and TO-CNCs was conducted via carbodiimide-assisted coupling chemistry. The synthesized TO-CNC@CQD hybrid nanomaterials were characterized using X-ray photoelectron spectroscopy, cryo-transmittance electron microscopy, confocal microscopy, and fluorescence spectroscopy. Finally, the interactions of TO-CNC@CQD hybrids with HeLa and RAW 264.7 macrophage cells were investigated in vitro. Cell viability tests suggest the surface conjugation with NH2-CQDs not only improved the cytocompatibility of TO-CNCs, but also enhanced their cellular association and internalization on both HeLa and RAW 264.7 cells after 4 and 24 h incubation.

UR - http://www.scopus.com/inward/record.url?scp=85022198372&partnerID=8YFLogxK

U2 - 10.1021/acs.biomac.7b00306

DO - 10.1021/acs.biomac.7b00306

M3 - Article

VL - 18

SP - 2045

EP - 2055

JO - Biomacromolecules

JF - Biomacromolecules

SN - 1525-7797

IS - 7

ER -

ID: 14386704