Performance and bacterial community dynamics of a CANON bioreactor acclimated from high to low operational temperatures

Alejandro Gonzalez-Martinez*, Alejandro Rodriguez-Sanchez, Maria Jesus Garcia-Ruiz, Babara Munoz-Palazon, Carmen Cortes-Lorenzo, Franscisco Osorio, Riku Vahala

*Corresponding author for this work

Research output: Contribution to journalArticleScientificpeer-review

83 Citations (Scopus)

Abstract

In this study, a lab-scale CANON bioreactor was operated for 260 days, decreasing operational temperature from 35 degrees C to 15 degrees C and from 466 to 100 mg-N L-1 ammonium. This was done in order to check the feasibility of the acclimation of CANON biomass treating anaerobic digestion supernatant to B-stage influent wastewater operational conditions. Results showed that decrease in temperature posed an impact over the performance of the CANON bioreactor as well as over its bacterial assemblage. Nitrogen removal efficiency showed a moderate decrease when the system was acclimated from 35 degrees C to 25 degrees C, but it decreased dramatically in the acclimation from 25 degrees C to 15 degrees C. The decrease in temperature and influent ammonium concentration posed an impact over the bacterial community structure of the system. Ammonium oxidizing bacteria changed from Nitrosomonas, Nitrosospira or Nitrosovibrio genera at high temperature and influent ammonium to Prosthecobacter at low temperature and low ammonium. As well, dominant anammox bacteria genus changed from Candidatus Brocadia to Candidatus Anammoxoglobus during acclimation. Proliferation of nitrite oxidizing bacteria only occurred under low temperature and influent ammonium conditions with growth of Rhodanobacter genus. (C) 2015 Elsevier B.V. All rights reserved.

Original languageEnglish
Pages (from-to)557-567
Number of pages11
JournalChemical Engineering Journal
Volume287
DOIs
Publication statusPublished - 1 Mar 2016
MoE publication typeA1 Journal article-refereed

Keywords

  • Anammox
  • CANON
  • Low temperature
  • Nitrogen removal
  • Pyrosequencing
  • Wastewater treatment
  • AUTOTROPHIC NITROGEN-REMOVAL
  • WASTE-WATER
  • SP-NOV.
  • PARTIAL NITRITATION/ANAMMOX
  • MEMBRANE BIOREACTOR
  • MICROBIAL COMMUNITY
  • OXIDIZING BACTERIA
  • LAB-SCALE
  • SYSTEM
  • NITRIFICATION

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