Paper-based plasmon-enhanced protein sensing by controlled nucleation of silver nanoparticles on cellulose

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Paper-based plasmon-enhanced protein sensing by controlled nucleation of silver nanoparticles on cellulose. / Arcot Raghupathi, Lokanathan; Uddin, Khan Mohammad Ahsan; Chen, Xi; Xiang, Wenchao; Kong, Xianming; Johansson, Leena S.; Ras, Robin H. A.; Rojas Gaona, Orlando.

In: Cellulose, Vol. 22, No. 6, 2015, p. 4027-4034.

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Arcot Raghupathi, Lokanathan ; Uddin, Khan Mohammad Ahsan ; Chen, Xi ; Xiang, Wenchao ; Kong, Xianming ; Johansson, Leena S. ; Ras, Robin H. A. ; Rojas Gaona, Orlando. / Paper-based plasmon-enhanced protein sensing by controlled nucleation of silver nanoparticles on cellulose. In: Cellulose. 2015 ; Vol. 22, No. 6. pp. 4027-4034.

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@article{69be9fd1895e4a758dbc70aea09ded76,
title = "Paper-based plasmon-enhanced protein sensing by controlled nucleation of silver nanoparticles on cellulose",
abstract = "Cheap, disposable bio-diagnostic devices are becoming increasingly prevalent in the field of biosensing. Earlier we had reported the ability of cellulosic surface to control the nucleation of plasmonic silver nanoparticles and in this report we utilize this nucleation controlling property to demonstrate a new plasmonic sensing mechanism based on paper substrates to quantitatively detect proteins. On contrary to conventional paper based diagnostic devices which use the cellulosic part of paper as a support structure, the proposed method takes advantage of cellulose as nucleation controller during silver nanoparticle formation. Reduction of silver ions interacting competitively with nucleation controlling cellulosic surface and reduction suppressing amino acids of protein (via complexation) resulted in silver nanoparticles whose size-shape dependent plasmonic property quantitatively reflected the concentration of protein on paper, characterized using UV-Vis and surface-enhanced Raman spectroscopies. As a proof-of-concept, bovine serum albumin (BSA) was tested as the target analyte. UV-Vis spectroscopy based BSA quantification was sensitive in the concentration range 10-60 mg ml(-1) while that for surface enhanced Raman spectroscopy extended well below 10 mg ml(-1), thus demonstrating the potential of this simple method to quantitatively detect a wide range of proteins relevant to the field of biodiagnostics.",
keywords = "Paper-biosensor, Cellulose-biosensor, Plasmonic-sensor, Biodiagnostics, RAMAN-SCATTERING, SURFACE, SENSOR, EAPAP",
author = "{Arcot Raghupathi}, Lokanathan and Uddin, {Khan Mohammad Ahsan} and Xi Chen and Wenchao Xiang and Xianming Kong and Johansson, {Leena S.} and Ras, {Robin H. A.} and {Rojas Gaona}, Orlando",
year = "2015",
doi = "10.1007/s10570-015-0783-z",
language = "English",
volume = "22",
pages = "4027--4034",
journal = "Cellulose",
issn = "0969-0239",
number = "6",

}

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TY - JOUR

T1 - Paper-based plasmon-enhanced protein sensing by controlled nucleation of silver nanoparticles on cellulose

AU - Arcot Raghupathi, Lokanathan

AU - Uddin, Khan Mohammad Ahsan

AU - Chen, Xi

AU - Xiang, Wenchao

AU - Kong, Xianming

AU - Johansson, Leena S.

AU - Ras, Robin H. A.

AU - Rojas Gaona, Orlando

PY - 2015

Y1 - 2015

N2 - Cheap, disposable bio-diagnostic devices are becoming increasingly prevalent in the field of biosensing. Earlier we had reported the ability of cellulosic surface to control the nucleation of plasmonic silver nanoparticles and in this report we utilize this nucleation controlling property to demonstrate a new plasmonic sensing mechanism based on paper substrates to quantitatively detect proteins. On contrary to conventional paper based diagnostic devices which use the cellulosic part of paper as a support structure, the proposed method takes advantage of cellulose as nucleation controller during silver nanoparticle formation. Reduction of silver ions interacting competitively with nucleation controlling cellulosic surface and reduction suppressing amino acids of protein (via complexation) resulted in silver nanoparticles whose size-shape dependent plasmonic property quantitatively reflected the concentration of protein on paper, characterized using UV-Vis and surface-enhanced Raman spectroscopies. As a proof-of-concept, bovine serum albumin (BSA) was tested as the target analyte. UV-Vis spectroscopy based BSA quantification was sensitive in the concentration range 10-60 mg ml(-1) while that for surface enhanced Raman spectroscopy extended well below 10 mg ml(-1), thus demonstrating the potential of this simple method to quantitatively detect a wide range of proteins relevant to the field of biodiagnostics.

AB - Cheap, disposable bio-diagnostic devices are becoming increasingly prevalent in the field of biosensing. Earlier we had reported the ability of cellulosic surface to control the nucleation of plasmonic silver nanoparticles and in this report we utilize this nucleation controlling property to demonstrate a new plasmonic sensing mechanism based on paper substrates to quantitatively detect proteins. On contrary to conventional paper based diagnostic devices which use the cellulosic part of paper as a support structure, the proposed method takes advantage of cellulose as nucleation controller during silver nanoparticle formation. Reduction of silver ions interacting competitively with nucleation controlling cellulosic surface and reduction suppressing amino acids of protein (via complexation) resulted in silver nanoparticles whose size-shape dependent plasmonic property quantitatively reflected the concentration of protein on paper, characterized using UV-Vis and surface-enhanced Raman spectroscopies. As a proof-of-concept, bovine serum albumin (BSA) was tested as the target analyte. UV-Vis spectroscopy based BSA quantification was sensitive in the concentration range 10-60 mg ml(-1) while that for surface enhanced Raman spectroscopy extended well below 10 mg ml(-1), thus demonstrating the potential of this simple method to quantitatively detect a wide range of proteins relevant to the field of biodiagnostics.

KW - Paper-biosensor

KW - Cellulose-biosensor

KW - Plasmonic-sensor

KW - Biodiagnostics

KW - RAMAN-SCATTERING

KW - SURFACE

KW - SENSOR

KW - EAPAP

U2 - 10.1007/s10570-015-0783-z

DO - 10.1007/s10570-015-0783-z

M3 - Article

VL - 22

SP - 4027

EP - 4034

JO - Cellulose

JF - Cellulose

SN - 0969-0239

IS - 6

ER -

ID: 1475882