Ordered nano-structure of a stamped self-organized protein layer on a HOPG surface using a HFB carrier

Research output: Contribution to journalArticleScientificpeer-review


  • Atsushi Iwanaga
  • Hitoshi Asakawa
  • Takeshi Fukuma
  • Momoka Nakamichi
  • Sakurako Shigematsu
  • Markus Linder

  • Tetsuya Haruyama

Research units

  • VTT Technical Research Centre of Finland
  • Kyushu Institute of Technology
  • Kanazawa University


A groundbreaking method for ordered molecular layer preparation on a solid surface employing the drop-stamp method has been developed by us taking advantage of the characteristics of the HFB molecule as a self-organizer/adsorption carrier. It is a smart method which can be used to prepare a self-organized protein layer on a solid surface without unspecific adsorption or defects. In our previous report, we clarified the self-organizing nature of HFB-tagged protein molecules on a surface of a solution droplet. In this report, a protein layer was prepared on a HOPG surface by using the drop-stamp method with a maltose binding protein (MBP)-tagged HFBII molecule. The structure of the stamped protein layer was investigated using frequency modulation atomic force microscopy (FM-AFM) in a liquid condition. The FM-AFM images show that the drop-stamp method can prepare an ordered protein layer on a solid surface smartly. The drop-stamp method using a HFB carrier is a practical method which can be used to prepare an ordered protein layer on a solid substrate surface without unspecific adsorption defects.


Original languageEnglish
Pages (from-to)395-399
Number of pages5
JournalColloids and Surfaces B: Biointerfaces
Issue number2
Publication statusPublished - 1 Jun 2011
MoE publication typeA1 Journal article-refereed

    Research areas

  • Drop-stamp, Hydrophobin, Molecular interface, Self-organized protein layer

ID: 4807987