Optimizing the step-by-step forming processes for fabricating a poly(DL -lactic-co-glycolic acid)-sandwiched cell/hydrogel construct

Xiaohong Wang*, Shaochun Sui, Chang Liu

*Corresponding author for this work

    Research output: Contribution to journalArticleScientificpeer-review

    17 Citations (Scopus)

    Abstract

    A poly(DL-lactic-co-glycolic acid) (PLGA)-sandwiched cell/fibrin construct was fabricated to overcome the weak mechanical properties of cell/hydrogel mixtures. This construct was formed with a step-by-step mold/extraction method to generate a middle smooth muscle layer of natural blood vessels. A desired three-layer construct, as an integrated entity with optimized inner structures, was achieved by the control of the size of the molds, the concentration of the polymer systems, and the temperature of the extraction and polymerization processes. The constructs were fabricated with the following dimensions: length = 10-25 mm, diameter >2 mm, and wall thickness = 0.6-2 mm. Different microstructures in different layers of the sandwiched structure resulted in different functions. The pore structure in the inner PLGA and middle fibrin layers was beneficial for nutrient transference, whereas the solid structure without pores in the outmost surface of the outer PLGA layer could prevent fluid from leaking during in vitro culturing and in vivo implantation. This study showed that this can be a promising approach for the fabrication of synthetic-polymer-sandwiched viable cell/hydrogel constructs for wide potential application in complex organ manufacturing.

    Original languageEnglish
    Pages (from-to)1199-1207
    Number of pages9
    JournalJournal of Applied Polymer Science
    Volume120
    Issue number2
    DOIs
    Publication statusPublished - 15 Apr 2011
    MoE publication typeA1 Journal article-refereed

    Keywords

    • biocompatibility
    • bioengineering
    • biological applications of polymers
    • biopolymers
    • injection molding

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