O-GlcNAcylation of the transcription machinery in prostate cancer cells

Research output: ThesisMaster's thesis


O-GlcNAcylation is a post-translational protein modification overexpressed in the most aggressive prostate cancer, which is the most common cancer type amongst men in Finland. O-GlcNAcylation has been found to play a role in gene transcription as an alternative protein modification to phosphorylation by transcription-associated cyclin-dependent kinases (TCDK). The aim of this thesis is to study the O-GlcNAcylation of transcriptional machinery in prostate cancer cells when inhibiting each TCDK activity. Cancer cells were first treated with on-target doses of TCDK inhibitors, followed by enrichment of O-GlcNAcyted proteins using lectin pulldown and investigating transcriptional machinery proteins levels by performing western blot. Results revealed a significant increase of CDK9 O-GlcNAcylation after inhibiting CDK9 activity in all used prostate cell lines. These findings highlight the important and yet not well-known role of O-GlcNAcylation in gene transcription in prostate cancer cells.
Original languageEnglish
QualificationMaster's degree
Awarding Institution
  • Aalto University
  • Frey, Alexander, Supervising Professor
  • Itkonen, Harri M., Thesis Advisor, External person
Award date20 Sept 2023
Publication statusPublished - 2021
MoE publication typeG2 Master's thesis, polytechnic Master's thesis


  • O-GlcNAcylation
  • transcription-associated cyclin-dependent kinases
  • hallmarks of cancer
  • prostate cancer


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