Nutritional requirements and strain heterogeneity in Ashbya gossypii

Orquidea Ribeiro, Lucilia Domingues, Merja Penttila, Marilyn G. Wiebe*

*Corresponding author for this work

Research output: Contribution to journalArticleScientificpeer-review

Abstract

Colony radial growth rates and specific growth rates of three related Ashbya gossypii strains ATCC10895, IMI31268, MUCL29450 and an unrelated strain, CBS109.26, were measured on various carbon and nitrogen sources at pH 4.5 and pH 6.5 to elucidate physiological growth requirements and strain differences. All strains grew on yeast extract or ammonium as nitrogen sources, but not on nitrate. Substantial growth at pH 4.5 was observed only on complex medium. D-Glucose, glycerol and starch were utilised as carbon sources. Ethanol was produced during growth on glycerol. Conversion of xylose into xylitol demonstrates that the xylose reductase is active. Phenotypic differences between related strains were greater than expected. We demonstrate that A. gossypii utilizes ammonium as sole nitrogen source at pH 6.5, facilitating further physiological studies using chemically defined media in the future. (C) 2012 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim)

Original languageEnglish
Pages (from-to)582-589
Number of pages8
JournalJOURNAL OF BASIC MICROBIOLOGY
Volume52
Issue number5
DOIs
Publication statusPublished - Oct 2012
MoE publication typeA1 Journal article-refereed

Keywords

  • Ashbya gossypii
  • Nitrogen utilization
  • Carbon utilization
  • Colony radial growth rate
  • Ethanol
  • pH
  • NADP-GLUTAMATE DEHYDROGENASE
  • SACCHAROMYCES-CEREVISIAE
  • RIBOFLAVIN PRODUCTION
  • AMMONIUM ASSIMILATION
  • NEMATOSPORA GOSSYPII
  • ISOCITRATE DEHYDROGENASE
  • ASPERGILLUS-NIDULANS
  • L-ARABINOSE
  • NRRL 1363
  • SYNTHASE

Cite this