Nile Red staining of phytoplankton neutral lipids: species-specific fluorescence kinetics in various solvents

Katariina Natunen; Jukka Seppälä; Dagmar Schwenk; Heiko Rischer; Kristian Spilling; Timo Tamminen

doi:10.1007/s10811-014-0404-5

Nile Red staining of phytoplankton neutral lipids: species-specific fluorescence kinetics in various solvents

Katariina Natunen^*, Jukka Seppälä, Dagmar Schwenk, Heiko Rischer, Kristian Spilling, Timo Tamminen

^*Corresponding author for this work

Not published at Aalto University

Research output: Contribution to journal › Article › Scientific › peer-review

18 Citations (Scopus)

Abstract

Nile Red (NR) staining potentially offers a simple method for monitoring lipid accumulation in microalgal cultivation. However, variable staining efficiencies and methods have been reported. The effect of dimethyl sulfoxide (DMSO), ethylene glycol (EG) and glycerol on NR penetration with four different phytoplankton species representing different taxonomical groups was studied. Treatment with the solvents enhanced the NR fluorescence of the diatom Phaeodactylum tricornutum during kinetic fluorescence measurements, but high concentrations of solvents were needed. None of the solvents improved NR staining of the green alga Chlorella pyrenoidosa and Scenedesmus obliquus, which are known to be difficult to stain due to their thick and rigid cell walls. The naked Isochrysis sp. cells stained best without solvents. The results confirm that NR staining protocol needs to be optimized for each species.

Original language	English
Pages (from-to)	1161-1168
Number of pages	8
Journal	JOURNAL OF APPLIED PHYCOLOGY
Volume	27
Issue number	3
DOIs	https://doi.org/10.1007/s10811-014-0404-5
Publication status	Published - 28 Jun 2015
MoE publication type	A1 Journal article-refereed

Keywords

Fluorescence kinetics
Neutral lipids
Nile Red
Phytoplankton
Solvents

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title = "Nile Red staining of phytoplankton neutral lipids: species-specific fluorescence kinetics in various solvents",

abstract = "Nile Red (NR) staining potentially offers a simple method for monitoring lipid accumulation in microalgal cultivation. However, variable staining efficiencies and methods have been reported. The effect of dimethyl sulfoxide (DMSO), ethylene glycol (EG) and glycerol on NR penetration with four different phytoplankton species representing different taxonomical groups was studied. Treatment with the solvents enhanced the NR fluorescence of the diatom Phaeodactylum tricornutum during kinetic fluorescence measurements, but high concentrations of solvents were needed. None of the solvents improved NR staining of the green alga Chlorella pyrenoidosa and Scenedesmus obliquus, which are known to be difficult to stain due to their thick and rigid cell walls. The naked Isochrysis sp. cells stained best without solvents. The results confirm that NR staining protocol needs to be optimized for each species.",

keywords = "Fluorescence kinetics, Neutral lipids, Nile Red, Phytoplankton, Solvents",

author = "Katariina Natunen and Jukka Sepp{\"a}l{\"a} and Dagmar Schwenk and Heiko Rischer and Kristian Spilling and Timo Tamminen",

year = "2015",

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language = "English",

volume = "27",

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Nile Red staining of phytoplankton neutral lipids : species-specific fluorescence kinetics in various solvents. / Natunen, Katariina; Seppälä, Jukka; Schwenk, Dagmar; Rischer, Heiko; Spilling, Kristian; Tamminen, Timo.

In: JOURNAL OF APPLIED PHYCOLOGY, Vol. 27, No. 3, 28.06.2015, p. 1161-1168.

Research output: Contribution to journal › Article › Scientific › peer-review

TY - JOUR

T1 - Nile Red staining of phytoplankton neutral lipids

T2 - species-specific fluorescence kinetics in various solvents

AU - Natunen, Katariina

AU - Seppälä, Jukka

AU - Schwenk, Dagmar

AU - Rischer, Heiko

AU - Spilling, Kristian

AU - Tamminen, Timo

PY - 2015/6/28

Y1 - 2015/6/28

N2 - Nile Red (NR) staining potentially offers a simple method for monitoring lipid accumulation in microalgal cultivation. However, variable staining efficiencies and methods have been reported. The effect of dimethyl sulfoxide (DMSO), ethylene glycol (EG) and glycerol on NR penetration with four different phytoplankton species representing different taxonomical groups was studied. Treatment with the solvents enhanced the NR fluorescence of the diatom Phaeodactylum tricornutum during kinetic fluorescence measurements, but high concentrations of solvents were needed. None of the solvents improved NR staining of the green alga Chlorella pyrenoidosa and Scenedesmus obliquus, which are known to be difficult to stain due to their thick and rigid cell walls. The naked Isochrysis sp. cells stained best without solvents. The results confirm that NR staining protocol needs to be optimized for each species.

AB - Nile Red (NR) staining potentially offers a simple method for monitoring lipid accumulation in microalgal cultivation. However, variable staining efficiencies and methods have been reported. The effect of dimethyl sulfoxide (DMSO), ethylene glycol (EG) and glycerol on NR penetration with four different phytoplankton species representing different taxonomical groups was studied. Treatment with the solvents enhanced the NR fluorescence of the diatom Phaeodactylum tricornutum during kinetic fluorescence measurements, but high concentrations of solvents were needed. None of the solvents improved NR staining of the green alga Chlorella pyrenoidosa and Scenedesmus obliquus, which are known to be difficult to stain due to their thick and rigid cell walls. The naked Isochrysis sp. cells stained best without solvents. The results confirm that NR staining protocol needs to be optimized for each species.

KW - Fluorescence kinetics

KW - Neutral lipids

KW - Nile Red

KW - Phytoplankton

KW - Solvents

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