Nature's recipe for splitting inteins

Aino Sesilja Aranko, Alexander Wlodawer, Hideo Iwaï*

*Corresponding author for this work

Research output: Contribution to journalReview ArticleScientificpeer-review

44 Citations (Scopus)

Abstract

Protein splicing in trans by split inteins has increasingly become a powerful protein-engineering tool for protein ligation, both in vivo and in vitro. Over 100 naturally occurring and artificially engineered split inteins have been reported for protein ligation using protein trans-splicing. Here, we review the current status of the reported split inteins in order to delineate an empirical or rational strategy for constructing new split inteins suitable for various applications in biotechnology and chemical biology.

Original languageEnglish
Pages (from-to)263-271
Number of pages9
JournalPROTEIN ENGINEERING DESIGN AND SELECTION
Volume27
Issue number8
DOIs
Publication statusPublished - 2014
MoE publication typeA2 Review article in a scientific journal

Keywords

  • protein trans-splicing
  • protein ligation
  • protein splicing
  • split intein

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