Abstract
Protein splicing in trans by split inteins has increasingly become a powerful protein-engineering tool for protein ligation, both in vivo and in vitro. Over 100 naturally occurring and artificially engineered split inteins have been reported for protein ligation using protein trans-splicing. Here, we review the current status of the reported split inteins in order to delineate an empirical or rational strategy for constructing new split inteins suitable for various applications in biotechnology and chemical biology.
Original language | English |
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Pages (from-to) | 263-271 |
Number of pages | 9 |
Journal | PROTEIN ENGINEERING DESIGN AND SELECTION |
Volume | 27 |
Issue number | 8 |
DOIs | |
Publication status | Published - 2014 |
MoE publication type | A2 Review article, Literature review, Systematic review |
Keywords
- protein trans-splicing
- protein ligation
- protein splicing
- split intein