Nanosilver–silica composite: Prolonged antibacterial effects and bacterial interaction mechanisms for wound dressings

Research output: Contribution to journalArticleScientificpeer-review

Standard

Nanosilver–silica composite : Prolonged antibacterial effects and bacterial interaction mechanisms for wound dressings. / Mosselhy, Dina A.; Granbohm, Henrika; Hynönen, Ulla; Ge, Yanling; Palva, Airi; Nordström, Katrina; Hannula, Simo Pekka.

In: Nanomaterials, Vol. 7, No. 9, 261, 06.09.2017.

Research output: Contribution to journalArticleScientificpeer-review

Harvard

APA

Vancouver

Author

Bibtex - Download

@article{79a75dd30d674d15b7c928415f4bd844,
title = "Nanosilver–silica composite: Prolonged antibacterial effects and bacterial interaction mechanisms for wound dressings",
abstract = "Infected superficial wounds were traditionally controlled by topical antibiotics until the emergence of antibiotic-resistant bacteria. Silver (Ag) is a kernel for alternative antibacterial agents to fight this resistance quandary. The present study demonstrates a method for immobilizing small-sized (~5 nm) silver nanoparticles on silica matrix to form a nanosilver–silica (Ag–SiO2) composite and shows the prolonged antibacterial effects of the composite in vitro. The composite exhibited a rapid initial Ag release after 24 h and a slower leaching after 48 and 72 h and was effective against both methicillin-resistant Staphylococcus aureus (MRSA) and Escherichia coli (E. coli). Ultraviolet (UV)-irradiation was superior to filter-sterilization in retaining the antibacterial effects of the composite, through the higher remaining Ag concentration. A gauze, impregnated with the Ag–SiO2 composite, showed higher antibacterial effects against MRSA and E. coli than a commercial Ag-containing dressing, indicating a potential for the management and infection control of superficial wounds. Transmission and scanning transmission electron microscope analyses of the composite-treated MRSA revealed an interaction of the released silver ions with the bacterial cytoplasmic constituents, causing ultimately the loss of bacterial membranes. The present results indicate that the Ag–SiO2 composite, with prolonged antibacterial effects, is a promising candidate for wound dressing applications.",
keywords = "Antibacterial effects, Composite, Mechanisms of action, Prolonged silver leaching, Silica, Silver nanoparticles, Wound dressings",
author = "Mosselhy, {Dina A.} and Henrika Granbohm and Ulla Hyn{\"o}nen and Yanling Ge and Airi Palva and Katrina Nordstr{\"o}m and Hannula, {Simo Pekka}",
year = "2017",
month = "9",
day = "6",
doi = "10.3390/nano7090261",
language = "English",
volume = "7",
journal = "Nanomaterials",
issn = "2079-4991",
publisher = "Multidisciplinary Digital Publishing Institute (MDPI)",
number = "9",

}

RIS - Download

TY - JOUR

T1 - Nanosilver–silica composite

T2 - Prolonged antibacterial effects and bacterial interaction mechanisms for wound dressings

AU - Mosselhy, Dina A.

AU - Granbohm, Henrika

AU - Hynönen, Ulla

AU - Ge, Yanling

AU - Palva, Airi

AU - Nordström, Katrina

AU - Hannula, Simo Pekka

PY - 2017/9/6

Y1 - 2017/9/6

N2 - Infected superficial wounds were traditionally controlled by topical antibiotics until the emergence of antibiotic-resistant bacteria. Silver (Ag) is a kernel for alternative antibacterial agents to fight this resistance quandary. The present study demonstrates a method for immobilizing small-sized (~5 nm) silver nanoparticles on silica matrix to form a nanosilver–silica (Ag–SiO2) composite and shows the prolonged antibacterial effects of the composite in vitro. The composite exhibited a rapid initial Ag release after 24 h and a slower leaching after 48 and 72 h and was effective against both methicillin-resistant Staphylococcus aureus (MRSA) and Escherichia coli (E. coli). Ultraviolet (UV)-irradiation was superior to filter-sterilization in retaining the antibacterial effects of the composite, through the higher remaining Ag concentration. A gauze, impregnated with the Ag–SiO2 composite, showed higher antibacterial effects against MRSA and E. coli than a commercial Ag-containing dressing, indicating a potential for the management and infection control of superficial wounds. Transmission and scanning transmission electron microscope analyses of the composite-treated MRSA revealed an interaction of the released silver ions with the bacterial cytoplasmic constituents, causing ultimately the loss of bacterial membranes. The present results indicate that the Ag–SiO2 composite, with prolonged antibacterial effects, is a promising candidate for wound dressing applications.

AB - Infected superficial wounds were traditionally controlled by topical antibiotics until the emergence of antibiotic-resistant bacteria. Silver (Ag) is a kernel for alternative antibacterial agents to fight this resistance quandary. The present study demonstrates a method for immobilizing small-sized (~5 nm) silver nanoparticles on silica matrix to form a nanosilver–silica (Ag–SiO2) composite and shows the prolonged antibacterial effects of the composite in vitro. The composite exhibited a rapid initial Ag release after 24 h and a slower leaching after 48 and 72 h and was effective against both methicillin-resistant Staphylococcus aureus (MRSA) and Escherichia coli (E. coli). Ultraviolet (UV)-irradiation was superior to filter-sterilization in retaining the antibacterial effects of the composite, through the higher remaining Ag concentration. A gauze, impregnated with the Ag–SiO2 composite, showed higher antibacterial effects against MRSA and E. coli than a commercial Ag-containing dressing, indicating a potential for the management and infection control of superficial wounds. Transmission and scanning transmission electron microscope analyses of the composite-treated MRSA revealed an interaction of the released silver ions with the bacterial cytoplasmic constituents, causing ultimately the loss of bacterial membranes. The present results indicate that the Ag–SiO2 composite, with prolonged antibacterial effects, is a promising candidate for wound dressing applications.

KW - Antibacterial effects

KW - Composite

KW - Mechanisms of action

KW - Prolonged silver leaching

KW - Silica

KW - Silver nanoparticles

KW - Wound dressings

UR - http://www.scopus.com/inward/record.url?scp=85028997256&partnerID=8YFLogxK

U2 - 10.3390/nano7090261

DO - 10.3390/nano7090261

M3 - Article

AN - SCOPUS:85028997256

VL - 7

JO - Nanomaterials

JF - Nanomaterials

SN - 2079-4991

IS - 9

M1 - 261

ER -

ID: 15915945