Projects per year
In early breast cancer progression, cancer cells invade through a nanoporous basement membrane (BM) as a first key step toward metastasis. This invasion is thought to be mediated by a combination of proteases, which biochemically degrade BM matrix, and physical forces, which mechanically open up holes in the matrix. To date, techniques that quantify cellular forces of BM invasion in 3D culture have been unavailable. Here, we developed cellular-force measurements for breast cancer cell invasion in 3D culture that combine multiple-particle tracking of force-induced BM-matrix displacements at the nanoscale, and magnetic microrheometry of localized matrix mechanics. We find that cancer-cell protrusions exert forces from picoNewtons up to nanoNewtons during invasion. Strikingly, the protrusions extension involves stepwise increases in force, in steps of 0.2 to 0.5 nN exerted from every 30 s to 6 min. Thus, this technique reveals previously unreported dynamics of force generation by invasive protrusions in cancer cells.
|Number of pages||9|
|Early online date||11 Aug 2022|
|Publication status||Published - 28 Sep 2022|
|MoE publication type||A1 Journal article-refereed|
FingerprintDive into the research topics of 'Nanoscale Tracking Combined with Cell-Scale Microrheology Reveals Stepwise Increases in Force Generated by Cancer Cell Protrusions'. Together they form a unique fingerprint.
- 1 Finished
R2B-IMMUNATE: Personalize immunotherapies in breast cancer
Kyyrä, J., Arasalo, O., Aryal, U., Kielosto, M., Srbova, L., Lanens, S., Lehtonen, A. & Mäntylä, V.
01/01/2021 → 31/12/2022
Project: Business Finland: New business from research ideas (TUTLI)