Nanofibrillar cellulose-alginate hydrogel coated surgical sutures as cell-carrier systems

Research output: Contribution to journalArticleScientificpeer-review

Researchers

  • Patrick Laurén
  • Petter Somersalo
  • Irina Pitkänen
  • Yan Ru Lou
  • Arto Urtti
  • Jouni Partanen

  • Jukka Seppälä

  • Mari Madetoja
  • Timo Laaksonen
  • Antti Mäkitie
  • Marjo Yliperttula

Research units

  • University of Eastern Finland
  • Made Consulting Ltd Oy
  • Tampere University of Technology
  • University of Helsinki
  • Aalto University

Abstract

Hydrogel nanomaterials, especially those that are of non-human and non-animal origins, have great potential in biomedical and pharmaceutical sciences due to their versatility and inherent soft-tissue like properties. With the ability to simulate native tissue function, hydrogels are potentially well suited for cellular therapy applications. In this study, we have fabricated nanofibrillar cellulose-alginate (NFCA) suture coatings as biomedical devices to help overcome some of the limitations related to cellular therapy, such as low cell survivability and distribution out of target tissue. The addition of sodium alginate 8% (w/v) increased the NFCA hydrogel viscosity, storage and loss moduli by slightly under one order of magnitude, thus contributing significantly to coating strength. Confocal microscopy showed nearly 100% cell viability throughout the 2-week incubation period within and on the surface of the coating. Additionally, typical morphologies in the dual cell culture of spheroid forming HepG2 and monolayer type SK-HEP-1 were observed. Twelve out of 14 NFCA coated surgical sutures remained intact during the suturing operation with various mice and rat tissue; however, partial peeling off was observed in 2 of the coated sutures. We conclude that NFCA suture coatings could perform as cell-carrier systems for cellular based therapy and post-surgical treatment.

Details

Original languageEnglish
Article numbere0183487
JournalPloS one
Volume12
Issue number8
Publication statusPublished - 1 Aug 2017
MoE publication typeA1 Journal article-refereed

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