Nanofibrillar cellulose hydrogel promotes three-dimensional liver cell culture

Madhushree Bhattacharya, Melina M. Malinen, Patrick Lauren, Yan-Rou Lou, Saara W. Kuisma, Liisa Kanninen, Martina Lille, Anne Corlu, Christiane GuGuen-Guillouzo, Olli Ikkala, Antti Laukkanen, Arto Urtti, Marjo Yliperttula

Research output: Contribution to journalArticleScientificpeer-review

311 Citations (Scopus)
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Over the recent years, various materials have been introduced as potential 3D cell culture scaffolds. These include protein extracts, peptide amphiphiles, and synthetic polymers. Hydrogel scaffolds without human or animal borne components or added bioactive components are preferred from the immunological point of view. Here we demonstrate that native nanofibrillar cellulose (NFC) hydrogels derived from the abundant plant sources provide the desired functionalities. We show 1) rheological properties that allow formation of a 3D scaffold in-situ after facile injection, 2) cellular biocompatibility without added growth factors, 3) cellular polarization, and 4) differentiation of human hepatic cell lines HepaRG and HepG2. At high shear stress, the aqueous NFC has small viscosity that supports injectability, whereas at low shear stress conditions the material is converted to an elastic gel. Due to the inherent biocompatibility without any additives, we conclude that NFC generates a feasible and sustained microenvironment for 3D cell culture for potential applications, such as drug and chemical testing, tissue engineering, and cell therapy.
Original languageEnglish
Pages (from-to)291-298
Number of pages8
JournalJournal of Controlled Release
Issue number3
Publication statusPublished - 2012
MoE publication typeA1 Journal article-refereed


  • Nanocellulose
  • 3D cell culture
  • Hepatocyte
  • Nanofiber
  • Plant-derived cellulose


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