Molecular cloning and enzymatic characterization of a Trichoderma reesei 1,2-alpha-D-mannosidase

Marleen Maras; Nico Callewaert; Kathleen Piens; Marc Claeyssens; Wim Martinet; Sylviane Dewaele; Hans Contreras; Isabelle Dewerte; Merja Penttilä; Roland Contreras

doi:10.1016/S0168-1656(99)00222-9

Molecular cloning and enzymatic characterization of a Trichoderma reesei 1,2-alpha-D-mannosidase

Marleen Maras, Nico Callewaert, Kathleen Piens, Marc Claeyssens, Wim Martinet, Sylviane Dewaele, Hans Contreras, Isabelle Dewerte, Merja Penttilä, Roland Contreras^*

^*Corresponding author for this work

Not published at Aalto University

Research output: Contribution to journal › Article › Scientific › peer-review

53 Citations (Scopus)

Abstract

A cDNA encoding 1,2-alpha-D-mannosidase mds1 from Trichoderma reesei was cloned. The largest open reading frame occupied 1571 bp. The predicted sequence contains 523 amino acid residues for a calculated molecular mass of 56 266 Da and shows high similarity to the amino acid sequences of 1,2-alpha-D-mannosidases from Aspergillus saitoi and Penicillium citrinum (51.6 and 51.0% identity, respectively). T. reesei mannosidase was produced as a recombinant enzyme in the yeast Pichia pastoris. Replacement of the N-terminal part with the prepro-signal peptide of the Saccharomyces cerevisiae alpha-mating factor resulted in high amounts of secreted enzyme. A three-step purification protocol was designed and the enzymatic properties were analysed. The enzyme was characterized as a class-I mannosidase. (C) 2000 Elsevier Science B.V. All rights reserved.

Original language	English
Pages (from-to)	255-263
Number of pages	9
Journal	Journal of Biotechnology
Volume	77
Issue number	2-3
DOIs	https://doi.org/10.1016/S0168-1656(99)00222-9
Publication status	Published - 17 Feb 2000
MoE publication type	A1 Journal article-refereed

Keywords

mannosidase
oligosaccharide
yeast
Trichoderma reesei
glycosidase
fungus
YEAST PICHIA-PASTORIS
ALPHA-MANNOSIDASE
ASPERGILLUS-SAITOI
GENE-EXPRESSION
SACCHAROMYCES-CEREVISIAE
PENICILLIUM-CITRINUM
NUCLEOTIDE-SEQUENCE
PROTEIN
OLIGOSACCHARIDES
GLYCOPROTEINS

Access to Document

10.1016/S0168-1656(99)00222-9

Cite this

@article{33c5aa299465413283a049bb7c5470dd,

title = "Molecular cloning and enzymatic characterization of a Trichoderma reesei 1,2-alpha-D-mannosidase",

abstract = "A cDNA encoding 1,2-alpha-D-mannosidase mds1 from Trichoderma reesei was cloned. The largest open reading frame occupied 1571 bp. The predicted sequence contains 523 amino acid residues for a calculated molecular mass of 56 266 Da and shows high similarity to the amino acid sequences of 1,2-alpha-D-mannosidases from Aspergillus saitoi and Penicillium citrinum (51.6 and 51.0% identity, respectively). T. reesei mannosidase was produced as a recombinant enzyme in the yeast Pichia pastoris. Replacement of the N-terminal part with the prepro-signal peptide of the Saccharomyces cerevisiae alpha-mating factor resulted in high amounts of secreted enzyme. A three-step purification protocol was designed and the enzymatic properties were analysed. The enzyme was characterized as a class-I mannosidase. (C) 2000 Elsevier Science B.V. All rights reserved.",

keywords = "mannosidase, oligosaccharide, yeast, Trichoderma reesei, glycosidase, fungus, YEAST PICHIA-PASTORIS, ALPHA-MANNOSIDASE, ASPERGILLUS-SAITOI, GENE-EXPRESSION, SACCHAROMYCES-CEREVISIAE, PENICILLIUM-CITRINUM, NUCLEOTIDE-SEQUENCE, PROTEIN, OLIGOSACCHARIDES, GLYCOPROTEINS",

author = "Marleen Maras and Nico Callewaert and Kathleen Piens and Marc Claeyssens and Wim Martinet and Sylviane Dewaele and Hans Contreras and Isabelle Dewerte and Merja Penttil{\"a} and Roland Contreras",

year = "2000",

month = feb,

day = "17",

doi = "10.1016/S0168-1656(99)00222-9",

language = "English",

volume = "77",

pages = "255--263",

journal = "Journal of Biotechnology",

issn = "0168-1656",

publisher = "Elsevier",

number = "2-3",

}

TY - JOUR

T1 - Molecular cloning and enzymatic characterization of a Trichoderma reesei 1,2-alpha-D-mannosidase

AU - Maras, Marleen

AU - Callewaert, Nico

AU - Piens, Kathleen

AU - Claeyssens, Marc

AU - Martinet, Wim

AU - Dewaele, Sylviane

AU - Contreras, Hans

AU - Dewerte, Isabelle

AU - Penttilä, Merja

AU - Contreras, Roland

PY - 2000/2/17

Y1 - 2000/2/17

N2 - A cDNA encoding 1,2-alpha-D-mannosidase mds1 from Trichoderma reesei was cloned. The largest open reading frame occupied 1571 bp. The predicted sequence contains 523 amino acid residues for a calculated molecular mass of 56 266 Da and shows high similarity to the amino acid sequences of 1,2-alpha-D-mannosidases from Aspergillus saitoi and Penicillium citrinum (51.6 and 51.0% identity, respectively). T. reesei mannosidase was produced as a recombinant enzyme in the yeast Pichia pastoris. Replacement of the N-terminal part with the prepro-signal peptide of the Saccharomyces cerevisiae alpha-mating factor resulted in high amounts of secreted enzyme. A three-step purification protocol was designed and the enzymatic properties were analysed. The enzyme was characterized as a class-I mannosidase. (C) 2000 Elsevier Science B.V. All rights reserved.

AB - A cDNA encoding 1,2-alpha-D-mannosidase mds1 from Trichoderma reesei was cloned. The largest open reading frame occupied 1571 bp. The predicted sequence contains 523 amino acid residues for a calculated molecular mass of 56 266 Da and shows high similarity to the amino acid sequences of 1,2-alpha-D-mannosidases from Aspergillus saitoi and Penicillium citrinum (51.6 and 51.0% identity, respectively). T. reesei mannosidase was produced as a recombinant enzyme in the yeast Pichia pastoris. Replacement of the N-terminal part with the prepro-signal peptide of the Saccharomyces cerevisiae alpha-mating factor resulted in high amounts of secreted enzyme. A three-step purification protocol was designed and the enzymatic properties were analysed. The enzyme was characterized as a class-I mannosidase. (C) 2000 Elsevier Science B.V. All rights reserved.

KW - mannosidase

KW - oligosaccharide

KW - yeast

KW - Trichoderma reesei

KW - glycosidase

KW - fungus

KW - YEAST PICHIA-PASTORIS

KW - ALPHA-MANNOSIDASE

KW - ASPERGILLUS-SAITOI

KW - GENE-EXPRESSION

KW - SACCHAROMYCES-CEREVISIAE

KW - PENICILLIUM-CITRINUM

KW - NUCLEOTIDE-SEQUENCE

KW - PROTEIN

KW - OLIGOSACCHARIDES

KW - GLYCOPROTEINS

U2 - 10.1016/S0168-1656(99)00222-9

DO - 10.1016/S0168-1656(99)00222-9

M3 - Article

SN - 0168-1656

VL - 77

SP - 255

EP - 263

JO - Journal of Biotechnology

JF - Journal of Biotechnology

IS - 2-3

ER -

Molecular cloning and enzymatic characterization of a Trichoderma reesei 1,2-alpha-D-mannosidase

Abstract

Keywords

Access to Document

Fingerprint

Cite this