Modes of action on cotton and bacterial cellulose of a homologous endoglucanase-exoglucanase pair from Trichoderma reesei

M Srisodsuk, K Kleman-Leyer, S Keranen, TK Kirk, TT Teeri*

*Corresponding author for this work

Research output: Contribution to journalArticleScientificpeer-review

Abstract

The endoglucanase I (EGI) and the cellobiohydrolase I (CBHI) of the filamentous fungus Trichoderma reesei form a homologous pair of cellulolytic enzymes which nevertheless have different modes of action, We show here that the action of CBHI on bacterial microcrystalline cellulose results in efficient solubilisation but only a slow decrease in its degree of polymerisation. In contrast, the action of EGI results in a rapid decrease of the degree of polymerisation but less efficient overall solubilisation of the substrate. CBHI alone was practically inactive toward cotton which has a high initial degree of polymerisation and a complex morphology. EGI rapidly reduced the degree of polymerisation of cotton, and slowly solubilised part of it Working synergistically, EGI and CBHI solubilised cotton more rapidly and to a greater extent than EGI alone. Our data are consistent with the exoglucanase nature of CBHI and also provide some evidence supporting its processive mode of action.

Original languageEnglish
Pages (from-to)885-892
Number of pages8
JournalEUROPEAN JOURNAL OF BIOCHEMISTRY
Volume251
Issue number3
Publication statusPublished - 1 Feb 1998
MoE publication typeA1 Journal article-refereed

Keywords

  • cellobiohydrolase
  • crystalline cellulose
  • endoglucanase
  • size-exclusion chromatography
  • synergy
  • CELLOBIOHYDROLASE-I
  • CRYSTALLINE CELLULOSE
  • BINDING DOMAIN
  • ENZYMATIC-HYDROLYSIS
  • CELLULASES
  • SYNERGISM
  • ADSORPTION
  • GENES
  • MECHANISM
  • FIMI

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