Modelling of ceramide interactions with porous graphite carbon in non-aqueous liquid chromatography

C. West, Geraldine Cilpa, K. Gaudin, P. Chaminade, E. Lesellier*

*Corresponding author for this work

Research output: Contribution to journalArticleScientificpeer-review

7 Citations (Scopus)

Abstract

Interactions of solutes on porous graphitic carbon (PGC) with non-aqueous mobile phases are studied by the linear solvation energy relationship (LSER). Studies have been carried out with eight binary mixtures composed of a weak solvent (acetonitrile or methanol) and a strong solvent (tetrahydrofuran, n-butanol, CH2Cl2, 1,1,2-trichloro-2,2,1-trifluoroethane). The systematic analysis of a set of test compounds was performed for each solvent mixture in isocratic mode (50:50). The results were compared to those obtained on PGC with hydro-organic liquids and supercritical fluids. They were then correlated with the observed retention behaviour of lipid compounds, more particularly ceramides.

Original languageEnglish
Pages (from-to)77-85
Number of pages9
JournalJOURNAL OF CHROMATOGRAPHY A
Volume1087
Issue number1-2
DOIs
Publication statusPublished - 16 Sep 2005
MoE publication typeA1 Journal article-refereed

Keywords

  • Ceramides
  • Linear solvation energy relationship (LSER)
  • Non-aqueous reversed-phase liquid chromatography (NARP-LC)
  • Porous graphitic carbon (PGC)

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