Microfluidic devices for biomolecular and cellular analysis

Ari Hokkanen

Research output: ThesisDoctoral ThesisCollection of Articles

Abstract

In this thesis microfluidic chips were developed for tissue sampling, chemical separation and electrical cell stimulation. Several fluorescence measurement systems were developed in order to enable small volume measurements in microchannels. Microneedle sampling was developed for cancer tissue analysis. A novel two channel silicon microneedle structure was designed and fabricated. Silicon chip was glue bonded to polymer microfluidic part, which had microfluidic channels as fluid reservoirs and actuation membranes for a fluidic control. Phosphatidylcholine (PC) and phosphoethanolamine (PE) lipids were successfully extracted as biomarkers for breast cancer. Microchip capillary electrophoresis (CE) was shown on silicon-glass chip, which had integrated thin film electrodes for high voltages (HV). This silicon-glass microchip was used for testosterone measurements with LIF detection. Another CE chip was made by hot embossing PMMA (polymethyl methacrylate). It was used to an antibiotic resistance measurement with mecA gene. Confocal four-colour laser induced fluorescence detection system was developed for roll-to-roll hot embossed PMMA chip. Glass chip was developed for human embryonic stem cells stimulation. Titanium thin film electrodes were deposited on glass surface. The native beat cycle of embryonic stem cells could be altered from about 1 s to 800 ms with electrical stimulation. Microfluidics makes fast healthcare analysis possible in doctor's office or even in home without time-consuming laboratory analysis. Treatment and medication could be started immediately without delay also avoiding unnecessary medication. Microfluidic devices require small sample amounts and low reagent consumption, which can lower cost of diagnosis; and tightly localized diagnosis and treatment, even at single cell level, enabling novel treatments. This is expected to bring major savings for healthcare system in future.
Translated title of the contributionMikrofluidistiikkalaitteita biomolekyylien ja solujen analysointiin
Original languageEnglish
QualificationDoctor's degree
Awarding Institution
  • Aalto University
Supervisors/Advisors
  • Franssila, Sami, Supervising Professor
  • Franssila, Sami, Thesis Advisor
Publisher
Print ISBNs978-952-60-6718-6
Electronic ISBNs978-952-60-6719-3
Publication statusPublished - 2016
MoE publication typeG5 Doctoral dissertation (article)

Keywords

  • microfluidics
  • microneedle
  • cell chip
  • capillary electrophoresis
  • fluorescence

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