Method with high-throughput screening potential for antioxidative substances using Escherichia coli biosensor katG'::lux

Jenni Tienaho; Tytti Sarjala; Robert Franzén; Matti Karp

doi:10.1016/j.mimet.2015.08.018

Method with high-throughput screening potential for antioxidative substances using Escherichia coli biosensor katG'::lux

Jenni Tienaho^*, Tytti Sarjala, Robert Franzén, Matti Karp

^*Corresponding author for this work

Not published at Aalto University

Research output: Contribution to journal › Article › Scientific › peer-review

11 Citations (Scopus)

Abstract

A new method is described for the rapid real-time screening of antioxidative properties using a recombinant Escherichia coli DPD2511 biosensor. This microplate technique, without time-consuming pre-incubations and handling, has potential for a high-throughput search of bioactive compounds. Special emphasis was given to obtaining highly reliable and repeatable results.

Original language	English
Pages (from-to)	78-80
Number of pages	3
Journal	Journal of Microbiological Methods
Volume	118
DOIs	https://doi.org/10.1016/j.mimet.2015.08.018
Publication status	Published - 1 Nov 2015
MoE publication type	A1 Journal article-refereed

Keywords

Antioxidative activity
Bacterial biosensor
Bioscreening
Microplate technique

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10.1016/j.mimet.2015.08.018

Cite this

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title = "Method with high-throughput screening potential for antioxidative substances using Escherichia coli biosensor katG'::lux",

abstract = "A new method is described for the rapid real-time screening of antioxidative properties using a recombinant Escherichia coli DPD2511 biosensor. This microplate technique, without time-consuming pre-incubations and handling, has potential for a high-throughput search of bioactive compounds. Special emphasis was given to obtaining highly reliable and repeatable results.",

keywords = "Antioxidative activity, Bacterial biosensor, Bioscreening, Microplate technique",

author = "Jenni Tienaho and Tytti Sarjala and Robert Franz{\'e}n and Matti Karp",

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AU - Tienaho, Jenni

AU - Sarjala, Tytti

AU - Franzén, Robert

AU - Karp, Matti

PY - 2015/11/1

Y1 - 2015/11/1

N2 - A new method is described for the rapid real-time screening of antioxidative properties using a recombinant Escherichia coli DPD2511 biosensor. This microplate technique, without time-consuming pre-incubations and handling, has potential for a high-throughput search of bioactive compounds. Special emphasis was given to obtaining highly reliable and repeatable results.

AB - A new method is described for the rapid real-time screening of antioxidative properties using a recombinant Escherichia coli DPD2511 biosensor. This microplate technique, without time-consuming pre-incubations and handling, has potential for a high-throughput search of bioactive compounds. Special emphasis was given to obtaining highly reliable and repeatable results.

KW - Antioxidative activity

KW - Bacterial biosensor

KW - Bioscreening

KW - Microplate technique

UR - http://www.scopus.com/inward/record.url?scp=84941101607&partnerID=8YFLogxK

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DO - 10.1016/j.mimet.2015.08.018

M3 - Article

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AN - SCOPUS:84941101607

SN - 0167-7012

VL - 118

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EP - 80

JO - Journal of Microbiological Methods

JF - Journal of Microbiological Methods

ER -

Method with high-throughput screening potential for antioxidative substances using Escherichia coli biosensor katG'::lux

Abstract

Keywords

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