Mature Let-7 miRNAs fine tune expression of LIN28B in pluripotent human embryonic stem cells

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Mature Let-7 miRNAs fine tune expression of LIN28B in pluripotent human embryonic stem cells. / Rahkonen, Nelly; Stubb, Aki; Malonzo, Maia; Edelman, Sanna; Emani, Maheswara Reddy; Närvä, Elisa; Lähdesmäki, Harri; Ruohola-Baker, Hannele; Lahesmaa, Riitta; Lund, Riikka.

In: STEM CELL RESEARCH, Vol. 17, No. 3, 01.11.2016, p. 498-503.

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Rahkonen, N, Stubb, A, Malonzo, M, Edelman, S, Emani, MR, Närvä, E, Lähdesmäki, H, Ruohola-Baker, H, Lahesmaa, R & Lund, R 2016, 'Mature Let-7 miRNAs fine tune expression of LIN28B in pluripotent human embryonic stem cells', STEM CELL RESEARCH, vol. 17, no. 3, pp. 498-503. https://doi.org/10.1016/j.scr.2016.09.025

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Rahkonen, Nelly ; Stubb, Aki ; Malonzo, Maia ; Edelman, Sanna ; Emani, Maheswara Reddy ; Närvä, Elisa ; Lähdesmäki, Harri ; Ruohola-Baker, Hannele ; Lahesmaa, Riitta ; Lund, Riikka. / Mature Let-7 miRNAs fine tune expression of LIN28B in pluripotent human embryonic stem cells. In: STEM CELL RESEARCH. 2016 ; Vol. 17, No. 3. pp. 498-503.

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@article{236640fd60d34617aec9420494f58640,
title = "Mature Let-7 miRNAs fine tune expression of LIN28B in pluripotent human embryonic stem cells",
abstract = "MicroRNAs (miRNA) are central regulators of diverse biological processes and are important in the regulation of stem cell self-renewal. One of the widely studied miRNA-protein regulators is the Lin28-Let-7 pair. In this study, we demonstrate that contrary to the well-established models of mouse ES cells (mESC) and transformed human cancer cells, the pluripotent state of human ES cells (hESC) involves expression of mature Let-7 family miRNAs with concurrent expression of all LIN28 proteins. We show that mature Let-7 miRNAs are regulated during hESC differentiation and have opposite expression profile with LIN28B. Moreover, mature Let-7 miRNAs fine tune the expression levels of LIN28B protein in pluripotent hESCs, whereas silencing of LIN28 proteins have no effect on mature Let-7 levels. These results bring novel information to the highly complex network of human pluripotency and suggest that maintenance of hESC pluripotency differs greatly from the mESCs in regard to LIN28-Let-7 regulation.",
keywords = "Human embryonic stem cell, Let-7, LIN28, Pluripotency",
author = "Nelly Rahkonen and Aki Stubb and Maia Malonzo and Sanna Edelman and Emani, {Maheswara Reddy} and Elisa N{\"a}rv{\"a} and Harri L{\"a}hdesm{\"a}ki and Hannele Ruohola-Baker and Riitta Lahesmaa and Riikka Lund",
year = "2016",
month = "11",
day = "1",
doi = "10.1016/j.scr.2016.09.025",
language = "English",
volume = "17",
pages = "498--503",
journal = "STEM CELL RESEARCH",
issn = "1873-5061",
publisher = "Elsevier",
number = "3",

}

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TY - JOUR

T1 - Mature Let-7 miRNAs fine tune expression of LIN28B in pluripotent human embryonic stem cells

AU - Rahkonen, Nelly

AU - Stubb, Aki

AU - Malonzo, Maia

AU - Edelman, Sanna

AU - Emani, Maheswara Reddy

AU - Närvä, Elisa

AU - Lähdesmäki, Harri

AU - Ruohola-Baker, Hannele

AU - Lahesmaa, Riitta

AU - Lund, Riikka

PY - 2016/11/1

Y1 - 2016/11/1

N2 - MicroRNAs (miRNA) are central regulators of diverse biological processes and are important in the regulation of stem cell self-renewal. One of the widely studied miRNA-protein regulators is the Lin28-Let-7 pair. In this study, we demonstrate that contrary to the well-established models of mouse ES cells (mESC) and transformed human cancer cells, the pluripotent state of human ES cells (hESC) involves expression of mature Let-7 family miRNAs with concurrent expression of all LIN28 proteins. We show that mature Let-7 miRNAs are regulated during hESC differentiation and have opposite expression profile with LIN28B. Moreover, mature Let-7 miRNAs fine tune the expression levels of LIN28B protein in pluripotent hESCs, whereas silencing of LIN28 proteins have no effect on mature Let-7 levels. These results bring novel information to the highly complex network of human pluripotency and suggest that maintenance of hESC pluripotency differs greatly from the mESCs in regard to LIN28-Let-7 regulation.

AB - MicroRNAs (miRNA) are central regulators of diverse biological processes and are important in the regulation of stem cell self-renewal. One of the widely studied miRNA-protein regulators is the Lin28-Let-7 pair. In this study, we demonstrate that contrary to the well-established models of mouse ES cells (mESC) and transformed human cancer cells, the pluripotent state of human ES cells (hESC) involves expression of mature Let-7 family miRNAs with concurrent expression of all LIN28 proteins. We show that mature Let-7 miRNAs are regulated during hESC differentiation and have opposite expression profile with LIN28B. Moreover, mature Let-7 miRNAs fine tune the expression levels of LIN28B protein in pluripotent hESCs, whereas silencing of LIN28 proteins have no effect on mature Let-7 levels. These results bring novel information to the highly complex network of human pluripotency and suggest that maintenance of hESC pluripotency differs greatly from the mESCs in regard to LIN28-Let-7 regulation.

KW - Human embryonic stem cell

KW - Let-7

KW - LIN28

KW - Pluripotency

UR - http://www.scopus.com/inward/record.url?scp=84994052361&partnerID=8YFLogxK

U2 - 10.1016/j.scr.2016.09.025

DO - 10.1016/j.scr.2016.09.025

M3 - Article

VL - 17

SP - 498

EP - 503

JO - STEM CELL RESEARCH

JF - STEM CELL RESEARCH

SN - 1873-5061

IS - 3

ER -

ID: 9309647