TY - JOUR
T1 - Macro-, micro-and nano-roughness of carbon-based interface with the living cells
T2 - Towards a versatile bio-sensing platform
AU - Golubewa, Lena
AU - Rehman, Hamza
AU - Kulahava, Tatsiana
AU - Karpicz, Renata
AU - Baah, Marian
AU - Kaplas, Tommy
AU - Shah, Ali
AU - Malykhin, Sergei
AU - Obraztsov, Alexander
AU - Rutkauskas, Danielis
AU - Jankunec, Marija
AU - Matulaitienė, Ieva
AU - Selskis, Algirdas
AU - Denisov, Andrei
AU - Svirko, Yuri
AU - Kuzhir, Polina
PY - 2020/9/2
Y1 - 2020/9/2
N2 - Integration of living cells with nonbiological surfaces (substrates) of sensors, scaffolds, and implants implies severe restrictions on the interface quality and properties, which broadly cover all elements of the interaction between the living and artificial systems (materials, surface modifications, drug-eluting coatings, etc.). Substrate materials must support cellular viability, preserve sterility, and at the same time allow real-time analysis and control of cellular activity. We have compared new substrates based on graphene and pyrolytic carbon (PyC) for the cultivation of living cells. These are PyC films of nanometer thickness deposited on SiO2 and black silicon and graphene nanowall films composed of graphene flakes oriented perpendicular to the Si substrate. The structure, morphology, and interface properties of these substrates are analyzed in terms of their biocompatibility. The PyC demonstrates interface biocompatibility, promising for controlling cell proliferation and directional intercellular contact formation while as-grown graphene walls possess high hydrophobicity and poor biocompatibility. By performing experiments with C6 glioma cells we discovered that PyC is a cell-friendly coating that can be used without poly-l-lysine or other biopolymers for controlling cell adhesion. Thus, the opportunity to easily control the physical/chemical properties and nanotopography makes the PyC films a perfect candidate for the development of biosensors and 3D bioscaffolds.
AB - Integration of living cells with nonbiological surfaces (substrates) of sensors, scaffolds, and implants implies severe restrictions on the interface quality and properties, which broadly cover all elements of the interaction between the living and artificial systems (materials, surface modifications, drug-eluting coatings, etc.). Substrate materials must support cellular viability, preserve sterility, and at the same time allow real-time analysis and control of cellular activity. We have compared new substrates based on graphene and pyrolytic carbon (PyC) for the cultivation of living cells. These are PyC films of nanometer thickness deposited on SiO2 and black silicon and graphene nanowall films composed of graphene flakes oriented perpendicular to the Si substrate. The structure, morphology, and interface properties of these substrates are analyzed in terms of their biocompatibility. The PyC demonstrates interface biocompatibility, promising for controlling cell proliferation and directional intercellular contact formation while as-grown graphene walls possess high hydrophobicity and poor biocompatibility. By performing experiments with C6 glioma cells we discovered that PyC is a cell-friendly coating that can be used without poly-l-lysine or other biopolymers for controlling cell adhesion. Thus, the opportunity to easily control the physical/chemical properties and nanotopography makes the PyC films a perfect candidate for the development of biosensors and 3D bioscaffolds.
KW - Biocompatibility
KW - Black silicon
KW - Glioma cells
KW - Graphene
KW - Graphene nanowalls
KW - Pyrolytic carbon
UR - http://www.scopus.com/inward/record.url?scp=85090184976&partnerID=8YFLogxK
U2 - 10.3390/s20185028
DO - 10.3390/s20185028
M3 - Article
C2 - 32899745
AN - SCOPUS:85090184976
VL - 20
SP - 1
EP - 14
JO - Sensors
JF - Sensors
SN - 1424-8220
IS - 18
M1 - 5028
ER -