Low pH D-xylonate production with Pichia kudriavzevii

Mervi Toivari*, Maija-Leena Vehkomaki, Yvonne Nygard, Merja Penttila, Laura Ruohonen, Marilyn G. Wiebe

*Corresponding author for this work

Research output: Contribution to journalArticleScientificpeer-review

Abstract

D-Xylonic acid is one of the top 30 most desirable chemicals to be derived from biomass sugars identified by the US Department of Energy, being applicable as a non-food substitute for D-gluconic acid and as a platform chemical. We engineered the non-conventional yeast Pichia kudriavzevii VU C-79090T to express a D-xylose dehydrogenase coding gene from Caulobacter crescentus. With this single modification the recombinant P. kudriavzevii strain produced up to 171 g L-1 of D-xylonate from 171 g L-1 D-xylose at a rate of 1.4 g L-1 h(-1) and yield of 1.0 g [g substrate consumed](-1), which was comparable with D-xylonate production by Gluconobacter oxydans or Pseudomonas sp. The productivity of the strain was also remarkable at low pH, producing 146 g L-1 D-xylonate at 1.2 g L-1 h(-1) at pH 3.0. This is the best low pH production reported for D-xylonate. These results encourage further development towards industrial scale production. (C) 2013 Elsevier Ltd. All rights reserved.

Original languageEnglish
Pages (from-to)555-562
Number of pages8
JournalBioresource Technology
Volume133
DOIs
Publication statusPublished - Apr 2013
MoE publication typeA1 Journal article-refereed

Keywords

  • Pichia kudriavzevii
  • Issatchenkia orientalis
  • D-Xylonate
  • D-Xylose
  • Low pH
  • LACTIC-ACID PRODUCTION
  • D-XYLOSE METABOLISM
  • SACCHAROMYCES-CEREVISIAE
  • ISSATCHENKIA-ORIENTALIS
  • MICROBIAL-PRODUCTION
  • CONTINUOUS-CULTURE
  • ESCHERICHIA-COLI
  • INTRACELLULAR PH
  • TOLERANCE
  • ETHANOL

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