Loss of NRF-2 and PGC-1α genes leads to retinal pigment epithelium damage resembling dry age-related macular degeneration

Research output: Contribution to journalArticleScientificpeer-review

Details

Original languageEnglish
Pages (from-to)1-12
Number of pages12
JournalRedox Biology
Volume20
StatePublished - 1 Jan 2019
MoE publication typeA1 Journal article-refereed

Researchers

  • Szabolcs Felszeghy
  • Johanna Viiri
  • Jussi J. Paterno
  • Juha M.T. Hyttinen
  • Ali Koskela
  • Mei Chen
  • Henri Leinonen
  • Heikki Tanila
  • Niko Kivinen
  • Arto Koistinen
  • Elisa Toropainen
  • Marialaura Amadio
  • Adrian Smedowski
  • Mika Reinisalo
  • Mateusz Winiarczyk
  • Jerzy Mackiewicz
  • Maija Mutikainen
  • Anna Kaisa Ruotsalainen
  • Mikko Kettunen
  • Kimmo Jokivarsi
  • Debasish Sinha
  • Kati Kinnunen
  • Goran Petrovski
  • Janusz Blasiak
  • Geir Bjørkøy
  • Heli Skottman
  • Arto Urtti
  • Antero Salminen
  • Ram Kannan
  • Deborah A. Ferrington
  • Heping Xu
  • Anna Liisa Levonen
  • Pasi Tavi
  • Anu Kauppinen
  • Kai Kaarniranta

Research units

  • University of Eastern Finland
  • Queen's University Belfast
  • University of Pavia
  • Medical University of Silesia in Katowice
  • University of Life Sciences in Lublin
  • Medical University of Lublin
  • Johns Hopkins University
  • University of Oslo
  • University of Lódz
  • Norwegian University of Science and Technology
  • University of Tampere
  • University of Helsinki
  • University of Southern California
  • University of Minnesota

Abstract

Age-related macular degeneration (AMD) is a multi-factorial disease that is the leading cause of irreversible and severe vision loss in the developed countries. It has been suggested that the pathogenesis of dry AMD involves impaired protein degradation in retinal pigment epithelial cells (RPE). RPE cells are constantly exposed to oxidative stress that may lead to the accumulation of damaged cellular proteins, DNA and lipids and evoke tissue deterioration during the aging process. The ubiquitin-proteasome pathway and the lysosomal/autophagosomal pathway are the two major proteolytic systems in eukaryotic cells. NRF-2 (nuclear factor-erythroid 2-related factor-2) and PGC-1α (peroxisome proliferator-activated receptor gamma coactivator-1 alpha) are master transcription factors in the regulation of cellular detoxification. We investigated the role of NRF-2 and PGC-1α in the regulation of RPE cell structure and function by using global double knockout (dKO) mice. The NRF-2/PGC-1α dKO mice exhibited significant age-dependent RPE degeneration, accumulation of the oxidative stress marker, 4-HNE (4-hydroxynonenal), the endoplasmic reticulum stress markers GRP78 (glucose-regulated protein 78) and ATF4 (activating transcription factor 4), and damaged mitochondria. Moreover, levels of protein ubiquitination and autophagy markers p62/SQSTM1 (sequestosome 1), Beclin-1 and LC3B (microtubule associated protein 1 light chain 3 beta) were significantly increased together with the Iba-1 (ionized calcium binding adaptor molecule 1) mononuclear phagocyte marker and an enlargement of RPE size. These histopathological changes of RPE were accompanied by photoreceptor dysmorphology and vision loss as revealed by electroretinography. Consequently, these novel findings suggest that the NRF-2/PGC-1α dKO mouse is a valuable model for investigating the role of proteasomal and autophagy clearance in the RPE and in the development of dry AMD.

    Research areas

  • Aging, Autophagy, Degeneration, Oxidative stress, Proteasome, Protein aggregation

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