TY - JOUR
T1 - Linkage of microbial kinetics and bacterial community structure of MBR and hybrid MBBR-MBR systems to treat salinity-amended urban wastewater
AU - Rodriguez-Sanchez, Alejandro
AU - Leyva-Diaz, Juan Carlos
AU - Gonzalez-Martinez, Alejandro
AU - Poyatos, Jose Manuel
PY - 2017
Y1 - 2017
N2 - Three pilot-scale bioreactors were started up and operated under salinity-amended urban wastewater feeding. The bioreactors were configured as membrane bioreactor and two different hybrid, moving bed biofilm reactor-membrane bioreactor and operated with a hydraulic retention time of 9.5 h, a solid residence time of 11.75 days and a total solids concentration of 2500 mg L-1. The three systems showed excellent performance in suspended solids, BOD5, and COD removal (values of 96-100%, 97-99%, and 88-90%, respectively), but poor nitrogen removal (values of 20-30%). The bacterial community structure during the start-up phase and the stabilization phase were different, as showed by β-diversity analyses. The differences between aerobic and anoxic biomass-and between suspended and attached biomass-were higher at the start-up phase than at the stabilization phase. The start-up phase showed high abundances of Chiayiivirga (mean values around 3-12% relative abundance) and Luteimonas (5-8%), but in the stabilization phase, the domination belonged to Thermomonas (3-14%), Nitrobacter (3-7%), Ottowia (3-11.5%), and Comamonas (2-6%), among others. Multivariate redundancy analyses showed that Thermomonas and Nitrosomonas were positively correlated with fast autotrophic kinetics, while Caulobacter and Ottowia were positively correlated with fast heterotrophic kinetics. Nitrobacter, Rhodanobacter, and Comamonas were positively correlated with fast autotrophic and heterotrophic kinetics.
AB - Three pilot-scale bioreactors were started up and operated under salinity-amended urban wastewater feeding. The bioreactors were configured as membrane bioreactor and two different hybrid, moving bed biofilm reactor-membrane bioreactor and operated with a hydraulic retention time of 9.5 h, a solid residence time of 11.75 days and a total solids concentration of 2500 mg L-1. The three systems showed excellent performance in suspended solids, BOD5, and COD removal (values of 96-100%, 97-99%, and 88-90%, respectively), but poor nitrogen removal (values of 20-30%). The bacterial community structure during the start-up phase and the stabilization phase were different, as showed by β-diversity analyses. The differences between aerobic and anoxic biomass-and between suspended and attached biomass-were higher at the start-up phase than at the stabilization phase. The start-up phase showed high abundances of Chiayiivirga (mean values around 3-12% relative abundance) and Luteimonas (5-8%), but in the stabilization phase, the domination belonged to Thermomonas (3-14%), Nitrobacter (3-7%), Ottowia (3-11.5%), and Comamonas (2-6%), among others. Multivariate redundancy analyses showed that Thermomonas and Nitrosomonas were positively correlated with fast autotrophic kinetics, while Caulobacter and Ottowia were positively correlated with fast heterotrophic kinetics. Nitrobacter, Rhodanobacter, and Comamonas were positively correlated with fast autotrophic and heterotrophic kinetics.
KW - Massive parallel sequencing
KW - Membrane bioreactor
KW - Microbial kinetics
KW - Moving bed biofilm reactor
KW - Salinity
KW - Wastewater
UR - http://www.scopus.com/inward/record.url?scp=85021076639&partnerID=8YFLogxK
U2 - 10.1002/btpr.2513
DO - 10.1002/btpr.2513
M3 - Article
AN - SCOPUS:85021076639
SN - 8756-7938
VL - 33
SP - 1483
EP - 1495
JO - Biotechnology Progress
JF - Biotechnology Progress
IS - 6
ER -